The potential auxiliary effects of Sargassum fusiform polysaccharides on sitagliptin in the treatment of diabetes mellitus

• Published in: International journal of biological macromolecules 2024-11, Vol.281 (Pt 1), p.136154, Article 136154
• Main Authors: Jia, Rui-Bo, Gao, Shang, Huang, Zirui, Li, Zhao-Rong, Wang, Haozheng, Wu, Juan, Zhou, Chunxia, Zhao, Mouming
• Format: Article
• Language: English
• Subjects: Animals; Auxiliary hypoglycemic effects; Blood Glucose - drug effects; Blood Glucose - metabolism; Diabetes Mellitus, Experimental - drug therapy; Feces - chemistry; Feces - microbiology; Gastrointestinal Microbiome - drug effects; Hypoglycemic Agents - chemistry; Hypoglycemic Agents - pharmacology; Insulin Resistance; Male; Polysaccharides - chemistry; Polysaccharides - pharmacology; Rats; Rats, Sprague-Dawley; Sargassum - chemistry; Sargassum fusiforme polysaccharides; Sitagliptin; Sitagliptin Phosphate - pharmacology; Underlying mechanism
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.136154

This work aimed to evaluate the potential positive effects of Sargassum fusiform polysaccharides (SFP) as add-on adjuncts to sitagliptin (SIT) in treating diabetes in rats. The results showed that both SIT and SIT co-administrated with SFP (SIT+SFP) could improve hyperglycemia, glucose tolerance, insulin resistance and hyperlipidemia, and SIT+SFP exhibited better effects in alleviating the levels of blood glucose, glucose tolerance, insulin resistance index, cholesterol, and low-density lipoprotein cholesterol compared to SIT administration. Intestinal flora analysis showed that SIT+SFP treatment significantly restored the beneficial composition of gut flora as compared with SIT administration, such as the increase of Lactobacillus, Romboutsia, Blautia, Bifidobacterium, Bacteroides, Ruminococcaceae_UCG_014 and Ruminococcus_1, and the decrease of Helicobacter, Escherichia-Shigella and Pseudomonas. The fecal metabolite analysis demonstrated that the fecal bile acid and short-chain fatty acid levels in the SIT+SFP group significantly increased compared to SIT treatment. Additionally, mRNA expression results confirmed that the hypoglycemic effects of SIT+SFP were better than those of SIT, which might be attributed to the regulation of blood glucose absorption, inhibition of gluconeogenesis and regulation of cholesterol metabolism. These results suggested that SFP could be used as an auxiliary substance for SIT in treating diabetes mellitus. [Display omitted] •SIT+SFP performed better antidiabetic effects in controlling FBG, HbA1c, AUC, HOMA-IRI, serum TC, LDL-C and FFA than SIT.•SIT+SFP contributed to an increase fecal SCFAs and BAs levels as compared with SIT.•SIT+SFP exhibited better regulatory effect on the expressions of InsR, IRS-1, PI3K, PPARγ, CYP7A1 and PEPCK than SIT.•SIT+SFP significantly restored beneficial composition of gut flora when compared with SIT.