Sulfated fucans from algae Saccharina japonica promotes intestinal stem cell-mediated intestinal development in juvenile mouse by modulating the gut microbiota

• Published in: International journal of biological macromolecules 2024-11, Vol.281 (Pt 2), p.136207, Article 136207
• Main Authors: Wei, Biqian, Ren, Pengfei, Qin, Wanting, Wang, Dehua, Wang, Yinfeng, Chang, Yaoguang, Wang, Yuming, Xue, Changhu, Tang, Qingjuan
• Format: Article
• Language: English
• Subjects: Animals; Edible Seaweeds; Gastrointestinal Microbiome - drug effects; Gut microbiota; Intestinal development; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestines - microbiology; Laminaria; Mice; Mice, Inbred C57BL; Mucin-2 - genetics; Mucin-2 - metabolism; Polysaccharides - chemistry; Polysaccharides - pharmacology; Stem Cells - drug effects; Stem Cells - metabolism; Sulfated fucans; Zonula Occludens-1 Protein - genetics; Zonula Occludens-1 Protein - metabolism
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.136207

Intestinal development has a crucial role in the absorption of nutrients and the ability to resist infections in the early stages of life. This study utilized a 3-week-old C57BL/6 mice model to evaluate the beneficial impacts of sulfated fucans from Saccharina japonica (SJ-FUC) on the growth and development of the intestines. SJ-FUC enhanced the dimensions of the intestine, specifically the length, height of villi, and depth of the crypts. Additionally, it raised the mRNA expression of ZO-1 and Occludin, hence enhancing the structural integrity of the intestinal epithelium. SJ-FUC significantly increased mRNA expression of Lyz1, Muc2, and Math1, which resulted in the promotion of intestinal epithelial development. Furthermore, SJ-FUC augmented the mRNA levels of the ISC markers (Lgr5, Olfm4, and Ascl2). Our further research uncovered that SJ-FUC has a positive impact on the growth of beneficial bacteria, such as Akkermansia, Dubosiella, and Lactobacillus, which in turn promotes epithelial development of the intestine. In summary, our research indicates that SJ-FUC has a beneficial impact on the growth of the intestines in young mice. This is achieved by enhancing the stemness of intestinal stem cells (ISCs) and promoting the formation of the intestinal epithelium through the regulation of gut bacteria.