Interaction of SARS-CoV-2 Spike protein with ACE2 induces cortical actin modulation, including dephosphorylation of ERM proteins and reduction of cortical stiffness

Cell surface cortical actin is a regulatory target for viral infection. We aimed to investigate the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on host cell cortical stiffness, an indicator of cortical actin structure. The receptor-binding domain (RBD) of SARS-Co...

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Bibliographic Details
Published in:Human cell : official journal of Human Cell Research Society 2024-10, Vol.38 (1), p.3, Article 3
Main Authors: Do, Thi Ly, Tachibana, Kouichi, Yamamoto, Norio, Ando, Kiyoshi, Isoda, Takaaki, Kihara, Takanori
Format: Article
Language:English
Subjects:
ACE2
Actin
Actins - metabolism
Angiotensin-converting enzyme 2
Angiotensin-Converting Enzyme 2 - metabolism
Angiotensin-Converting Enzyme 2 - physiology
Biomedical and Life Sciences
Cell Biology
Cell surface
Coronaviruses
COVID-19
Cytoskeletal Proteins - metabolism
Dephosphorylation
Ezrin
Gynecology
Humans
Life Sciences
Membrane Proteins - metabolism
Microfilament Proteins - metabolism
Moesin
Oncology
Phosphorylation
Protein Binding
Protein Domains
Radixin
Reproductive Medicine
Research Article
SARS-CoV-2 - physiology
Severe acute respiratory syndrome coronavirus 2
Spike Glycoprotein, Coronavirus - metabolism
Spike Glycoprotein, Coronavirus - physiology
Spike protein
Stem Cells
Surgery
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Summary:Cell surface cortical actin is a regulatory target for viral infection. We aimed to investigate the effect of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection on host cell cortical stiffness, an indicator of cortical actin structure. The receptor-binding domain (RBD) of SARS-CoV-2 Spike (S) protein induced a reduction in cortical stiffness in ACE2-expressing cells. The interaction of RBD with ACE2 caused the inactivation of Ezrin/Radixin/Moesin (ERM) proteins. We further investigated the effects of the RBD of SARS-CoV-2 Omicron variants, BA.1 and BA.5. These RBDs influenced cortical stiffness depending on their affinity for ACE2. Our study provides the first evidence that the interaction of the SARS-CoV-2 S protein with ACE2 induces mechanobiological signals and attenuates the cortical actin.
ISSN:1749-0774
0914-7470
1749-0774
DOI:10.1007/s13577-024-01142-2