Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography

• Published in: Archives of oral biology 2025-01, Vol.169, p.106111, Article 106111
• Main Authors: Uyar, Didem Sakaryalı, Karslıoğlu, Hazal, Ocak, Mert, Çelik, Hakan Hamdi
• Format: Article
• Language: English
• Subjects: Dental Pulp - diagnostic imaging; Dental Pulp - pathology; Dentin - diagnostic imaging; Dentin - pathology; Dentin mineral density; Humans; Imaging, Three-Dimensional - methods; In Vitro Techniques; Micro-computed tomography; Molar - diagnostic imaging; Molar incisor hypomineralization; Pulp stone; Pulp volume; X-Ray Microtomography - methods
• ISSN: 0003-9969; 1879-1506; 1879-1506
• DOI: 10.1016/j.archoralbio.2024.106111

To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth. This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p < 0.05. There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p < 0.05) and middle (p = 0.010; p < 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p < 0.001; p < 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p < 0.05). There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth. •Dentin mineral density was lower in hypomineralized teeth than healthy teeth.•Accessory canals were more common in cervical and middle of hypomineralized teeth.•Pulp stones were more in cervical of healthy teeth than hypomineralized teeth.•No difference was observed regarding pulp volume and microcracks between groups.