A simple method to determine proteolytic activity of snake venoms

In this work, we describe an easy, simple, and cost-effective method to assess the proteolytic activity of snake venoms. The method is based on measuring the hydrolytic halo formed by gelatin radial hydrolysis following the incubation of venoms on a solid gelatin-agarose plate. Venoms from Bothrops...

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Bibliographic Details
Published in:Toxicon (Oxford) 2024-11, Vol.251, p.108157, Article 108157
Main Authors: Camicia, Federico, Miguez, Rocío I., Lago, Néstor R., Damin, Carlos F., de Roodt, Adolfo R.
Format: Article
Language:English
Subjects:
Gelatinolytic activity
Hemorrhage
Snake venom
Snake venom metalloproteinases
Venom neutralization
Venom testing
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Summary:In this work, we describe an easy, simple, and cost-effective method to assess the proteolytic activity of snake venoms. The method is based on measuring the hydrolytic halo formed by gelatin radial hydrolysis following the incubation of venoms on a solid gelatin-agarose plate. Venoms from Bothrops (B.) alternatus, B. diporus, B. neuwiedi, B. jararaca, B. jararacussu, Crotalus atrox, and Trimeresurus albolabris were tested. A dose-response relationship was observed for each venom tested, with proteolytic capacity values, determined as GD (gelatinolytic dose, the dose causing a 15 mm hydrolytic halo) ranging from 21 to 222 μg. A correlation between hydrolysis and hemorrhagic activity in rat skin (minimal hemorrhagic dose) was found, with an r2 value of 0.8774 (p 
ISSN:0041-0101
1879-3150
1879-3150
DOI:10.1016/j.toxicon.2024.108157