Novel analytical approach for baclofen quantification in rodent plasma

A simple, accurate, and robust reverse‐phase high‐performance liquid chromatography (RP‐HPLC) method was developed and validated for the quantification of Baclofen in rat plasma. The method demonstrated high degree of linearity (r2 = 0.9936) across a concentration range of 10–50 μg/mL. Precision, ac...

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Bibliographic Details
Published in:Biomedical chromatography 2025-01, Vol.39 (1), p.e6038-n/a
Main Authors: Dhiman, Bindu, Yadav, Mithlesh, Dhiman, Anju, Satija, Saurabh
Format: Article
Language:English
Subjects:
Animals
baclofen
Baclofen - blood
Baclofen - chemistry
Baclofen - pharmacokinetics
bioavailability
Chromatography, High Pressure Liquid - methods
Chromatography, Reverse-Phase - methods
Limit of Detection
Linear Models
Male
method validation
pharmacokinetics
plasma
Rats
Rats, Sprague-Dawley
Reproducibility of Results
RP‐HPLC
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Description
Summary:A simple, accurate, and robust reverse‐phase high‐performance liquid chromatography (RP‐HPLC) method was developed and validated for the quantification of Baclofen in rat plasma. The method demonstrated high degree of linearity (r2 = 0.9936) across a concentration range of 10–50 μg/mL. Precision, accuracy, limit of detection (LOD), limit of quantification (LOQ), and robustness were evaluated according to ICH guidelines. The LOD and LOQ were found to be 0.076197 and 0.23090 μg/mL, respectively. This method provides an efficient approach for Baclofen quantification in plasma, making it suitable for pharmacokinetic and bioavailability studies. The novelty of this study lies in its optimization for routine use in laboratories, ensuring reproducibility with minimal variations across different conditions and analysts.
ISSN:0269-3879
1099-0801
1099-0801
DOI:10.1002/bmc.6038