Metabolic Versatility of acetogens in syngas Fermentation: Responding to varying CO availability

• Published in: Bioresource technology 2025-02, Vol.417, p.131823, Article 131823
• Main Authors: Moreira, João P.C., Domingues, Lucília, Alves, Joana I.
• Format: Article
• Language: English
• Subjects: Acetates - metabolism; Acetobacterium - metabolism; Acetobacterium wieringae; Carbon Dioxide - metabolism; Carbon Monoxide; Carbon Monoxide - metabolism; Clostridium - metabolism; Clostridium autoethanogenum; Clostridium carboxidivorans; Fermentation - physiology; Gases - metabolism; Hydrogen - metabolism; Kinetics; Syngas
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2024.131823

[Display omitted] •Varying CO levels in syngas affect H2 consumption, growth, and acetate production.•Acetobacterium wieringae JM exhibits the highest growth rate, 0.104 h−1.•Clostridium autoethanogenum co-utilizes CO, H2, and CO2 in all syngas compositions.•Syngas composition influences acetate production stoichiometry. Syngas fermentation using acetogenic bacteria offers a promising route for sustainable chemical production. However, gas–liquid mass transfer limitations and efficient co-utilization of CO and H2 pose significant challenges. This study investigated the kinetics of syngas conversion to acetate by Acetobacterium wieringae and Clostridium species in batch conditions under varying initial CO partial pressures (19 – 110 kPa). A. wieringae strains, exhibited superior growth in all gas compositions, with a maximum growth rate of 0.104 h−1. The distinct CO, H2, and CO2 consumption patterns revealed metabolic flexibility and adaptation to varying syngas compositions. Notably, A. wieringae strains and C. autoethanogenum achieved complete CO and H2 conversion, with C. autoethanogenum also exhibiting net CO2 uptake. These findings provide valuable insights into the distinct metabolic capabilities of these acetogens and contribute to the development of efficient and sustainable syngas fermentation processes.