Light pollution disrupts seasonal reproductive phenotypes and reduces lifespan in the West Nile vector, Culex pipiens

• Published in: Journal of insect physiology 2024-12, Vol.159, p.104725, Article 104725
• Main Authors: Fiorta, Maria A., Fyie, Lydia R., Meuti, Megan E.
• Format: Article
• Language: English
• Subjects: Animals; Artificial Light at Night; Behavioral phenotypes; Culex - physiology; Diapause; Feeding Behavior; Female; Fertility - radiation effects; Light; Longevity - radiation effects; Mosquito Vectors - physiology; Mosquito Vectors - radiation effects; Northern house mosquito; Phenotype; Photoperiod; Reproduction; Seasons; Urbanization
• ISSN: 0022-1910; 1879-1611; 1879-1611
• DOI: 10.1016/j.jinsphys.2024.104725

[Display omitted] •Artificial Light at Night (ALAN) adversely affects daily and seasonal responses in animals.•Culex pipiens bite and lay eggs in long days and enter an overwintering diapause during short days.•ALAN exposure decreased bloodfeeding but increased the fecundity of long-day reared females.•ALAN exposure did not affect the lifespan of long day-reared female mosquitoes.•ALAN exposure dramatically reduced the lifespan of short-day reared mosquitoes. Females of the Northern House mosquito, Culex pipiens, are important disease vectors as they transmit pathogens including West Nile virus. These females survive the winter by entering diapause, a state of dormancy, characterized by the accumulation of lipids, cessation of blood-feeding, and reproductive arrest. Diapause is cued by photoperiod, so as days become short in late summer and early fall, female Cx. pipiens prepare to overwinter and disease transmission decreases. We previously demonstrated that Artificial light at night (ALAN) causes female Cx. pipiens to avert diapause and continue to blood-feed when reared under short-day conditions. Additionally, light pollution alters seasonal differences in mosquito activity and nutrient reserves. However, it is unclear how exposure to ALAN affects blood-feeding and fecundity in long-day reared females, as well as the survival of Cx. pipiens exposed under both short and long-day conditions. In this study, we hypothesized that females exposed to ALAN in long-day conditions would have a lower proclivity to blood-feed, reduced fecundity, and reduced survival. Results from our lab-based experiments demonstrate that females exposed to ALAN in long-day conditions were less likely to blood-feed but were more fecund than long-day reared females that were not exposed to ALAN, and that ALAN exposure did not affect lifespan of long-day reared females. Additionally, we hypothesized ALAN exposure under short-day conditions would reduce survival, and our data supports this hypothesis. Overall, our results demonstrate that ALAN is an important urban stressor that has the potential to affect reproduction and lifespan in mosquitoes, and therefore has the potential to create evolutionary tradeoffs.