Effects of high-intensity interval training and moderate-intensity continuous training on neural dynamics and firing in the CA1-MEC region of mice

• Published in: Journal of applied physiology (1985) 2025-01, Vol.138 (1), p.31
• Main Authors: Liu, Yuncheng, Chen, Shiqiang, Li, Junliang, Song, Zengfei, Wang, Jihui, Ren, Xiping, Qian, Yongdong, Ouyang, Wei
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Animals; CA1 Region, Hippocampal - physiology; Entorhinal Cortex - physiology; High-Intensity Interval Training - methods; Male; Mice; Mice, Inbred ICR; Neurons - physiology; Physical Conditioning, Animal - methods; Physical Conditioning, Animal - physiology
• ISSN: 8750-7587; 1522-1601; 1522-1601
• DOI: 10.1152/japplphysiol.00778.2024

The aim of this study is to investigate the differential impacts of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on neural circuit dynamics and neuronal firing in the hippocampal CA1 subregion (CA1) region and medial entorhinal cortex (MEC) of mice. Forty-two male ICR mice were randomized into control, HIIT, and MICT groups. Electrophysiological recordings were performed pre- and postintervention to assess neural circuit dynamics and neuronal firing patterns in the CA1-MEC pathway. Both exercise protocols increased local field potential (LFP) coherence, with MICT showing a more pronounced effect on δ and γ coherences ( < 0.05). Both modalities reduced δ power spectral density (PSD) (HIIT, < 0.05; MICT, < 0.01) and elevated θ, β, and γ PSDs. Neuronal firing frequency improved in both CA1 and MEC following HIIT and MICT ( < 0.05). HIIT enhanced firing regularity in CA1 ( < 0.05), whereas MICT improved regularity in both regions ( < 0.05). Both protocols reduced firing latency (HIIT, < 0.05; MICT, < 0.01) and enhanced burst firing ratio, interburst interval (IBI), burst duration (BD), and LFP phase locking ( < 0.05 or < 0.01). Notably, MICT significantly improved spatial working memory and novel recognition abilities, as evidenced by increased novel arm time, entries, and preference index ( < 0.01). This study reveals that both HIIT and MICT positively impact neural processing and information integration in the CA1-MEC network of mice. Notably, MICT exhibits a more pronounced impact on neural functional connectivity and cognitive function compared with HIIT. These findings, coupled with the similarities in hippocampal electrophysiological characteristics between rodents and humans, suggest potential exercise-mediated neural plasticity and cognitive benefits in humans. This study is the first to investigate HIIT and MICT's effects on neural activity in the mouse CA1-MEC circuit, demonstrating that exercise modulates processing, enhances integration, and boosts cognitive performance. Due to similar hippocampal electrophysiology in rodents and humans during movement and navigation, our findings suggest implications for human brain neural changes, advancing the understanding of neurophysiological mechanisms underlying exercise-cognition interactions and informing exercise recommendations for cognitive health.