Grifola frondosa extract as a fetal bovine serum supplement for the culture of bovine muscle satellite cells under low serum conditions

• Published in: Food research international 2024-12, Vol.197 (Pt 1), p.115173, Article 115173
• Main Authors: Yu, In-sun, Choi, Sang Yoon, Choi, Jungseok, Kim, Mina K., Um, Min Young, Ahn, Jae Hwan, Kim, Min Jung
• Format: Article
• Language: English
• Subjects: Animals; Bovine muscle satellite cell; Cattle; Cell culture media; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cells, Cultured; Cultivated meat; Culture Media; Differentiation; Grifola - chemistry; Grifola frondosa; Hanwoo satellite cell; Muscle Development - drug effects; Plant Extracts - pharmacology; Proliferation; Satellite Cells, Skeletal Muscle - drug effects; Satellite Cells, Skeletal Muscle - metabolism; Serum
• ISSN: 0963-9969; 1873-7145; 1873-7145
• DOI: 10.1016/j.foodres.2024.115173

[Display omitted] •GFE at 12.5 μg/mL in low serum enhanced Hanwoo satellite cell proliferation and differentiation more than the positive control.•GFE significantly promoted the expression of myogenesis-related biomarkers.•GFE in low serum increased protein production and reduced total costs by 2.67–2.87 times compared to the control groups.•GFE has the potential to reduce FBS in culture medium for cultivated meat production. Expensive fetal bovine serum (FBS) is a major obstacle to the production of cultivated meat. However, because FBS substitutes do not sufficiently induce cell proliferation, a good alternative is to reduce the amount of FBS and use ingestible additives to promote cell proliferation. In this study, Grifola frondosa extract (GFE) was used to investigate its potential as an additive to promote myogenesis of bovine muscle satellite cells from Hanwoo cattle under low serum conditions (10 % FBS). GFE treated with 10 % FBS only during the proliferation period not only increased cell proliferation and related biomarkers in a concentration-dependent manner (0.78–12.5 μg/mL), but also increased cell differentiation. Additionally, differentiation was promoted when cells were with GFE treated only during the differentiation period. Especially GFE at 12.5 µg/mL induced significantly higher proliferation and differentiation rates than 20 % FBS medium. In particular, compared to treatment alone in the proliferation or differentiation periods, GFE treatment in both periods contributed to an increase in the differentiation rate and significantly enhanced total protein production. The integration of GFE into cultivated meat production presents a promising approach to reducing FBS dependence, lowering costs, and enhancing scalability, aligning with sustainability and consumer acceptance goals.