Role of intracellular storage polymers in simultaneous biological nutrient removal and resources recovery

• Published in: Journal of environmental management 2024-12, Vol.373, p.123720, Article 123720
• Main Authors: Srivastava, Ghazal, Aboudi, Kaoutar, Tyagi, Vinay Kumar, Kazmi, Absar Ahmad
• Format: Article
• Language: English
• Subjects: Biological nutrient removal; Enhanced biological phosphorus removal; Poly-β-hydroxybutyrates; Polyhydroxyalkanoates; Simultaneous nitrification and denitrification
• ISSN: 0301-4797; 1095-8630; 1095-8630
• DOI: 10.1016/j.jenvman.2024.123720

Simultaneous biological nutrient removal (SBNR) using an anaerobic-anoxic-oxic phase is the key feature of advanced wastewater treatment plants (WWTPs). Removing ammonia, total nitrogen, and phosphorus concurrently with organic matter and suspended solids from wastewater is essential to meeting stringent effluent discharge standards via SBNR in WWTPs. More insight into the mechanisms of SBNR, i.e., simultaneous nitrification-denitrification (SND) and enhanced biological phosphorus removal (EBPR) processes, the intracellular carbon reserves, i.e., polyhydroxyalkanoates (PHA) and specifically poly-β-hydroxybutyrates (PHB), will play a critical role in nutrients removal and resource recovery in WWTPs. Volatile fatty acids (VFA) in wastewater are the preferable source of PHA formation. However, municipal wastewater could not supply sufficient VFA fractions owing to short sewer lines; therefore, developing pre-fermentation chambers and other technological integration in the WWTPs can play an effective role in VFA production from raw sewage, resulting in the effective formation of PHA. On the other hand, PHA is a value-added biochemical, i.e., a potential substitute for fossil fuel plastics. WWTPs complying with SBNR are the bio-refineries for PHA (bioplastic precursors) production using diverse microbial populations. This review enlightens three dimensions of progressive systems and engineering-based viewpoints: (i) Increasing the SBNR by optimizing operational conditions subject to the substrate storage mechanisms of treatment systems; (ii) Technical solutions to enhance the VFA availability in sewage in WWTPs to achieve effective SBNR; and (iii) production of PHB (PHA) in WWTPs. [Display omitted] •Integrating nutrient removal with biopolymers recovery in sewage treatment plants is promising.•Polyhydroxyalkanoates are intracellular biopolymers playing a crucial role in nutrient removal.•Poly-β-hydroxybutyrates are electron donors for simultaneous nitrification-denitrification.•Volatile fatty acids are preferable carbon sources for poly-β-hydroxybutyrates accumulation.