In vitro study of dimethyl glutamate incorporated chitosan/microfibrillated cellulose based matrix in addition of H and Zr on osteoblast cells

• Published in: International journal of biological macromolecules 2024-12, Vol.289, p.138889, Article 138889
• Main Authors: Sivasankar, M.V., Sreenivasa Rao, P.
• Format: Article
• Language: English
• Subjects: Cellulose microfiber; Chitosan; Cytotoxicity; DMG; Hydroxyapatite; MG63 cells; Zirconium oxide
• ISSN: 0141-8130; 1879-0003; 1879-0003
• DOI: 10.1016/j.ijbiomac.2024.138889

Tissue engineering techniques can be utilized to repair or regenerate damaged tissue by promoting the proliferation and differentiation of cells in bone regeneration. A critical component of this process is the scaffold employed, which should ideally support consistent tissue development during bone regeneration. The aim of this study was to evaluate the morphological, physicochemical, and biological characteristics of various scaffolds: S1 (C/MFC), S2 (C/H/MFC), S3 (C/MFC/Zr), S4 (C/MFC/PCL), S5 (C/H/MFC/PCL), S6 (C/PCL/MFC/Zr), and S7 (C/H/MFC/Zr), which are intended for application in bone regeneration. The scaffolds containing microfibrillated cellulose, chitosan, polycaprolactone, zirconium, and hydroxyapatite were fabricated by the freeze-drying method. Conventional methods, including scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) analysis, were used to evaluate morphological and physicochemical properties of composite scaffolds. The fabricated scaffolds (S1-S7) had spongy properties that all functional groups were present in the sponge. Biological properties for cell survival were evaluated by the MTT assay, ALP, and ARS activities, respectively. In physicochemical studies, scaffolds showed tunable water absorption, swelling studies, degradation, sustained drug release, and mechanical properties. In biological studies, the cell proliferation and attachment were shown to significantly increase in scaffolds on MG63 cells. After 7 days of cell culture, ALP and ARS activity indicated the enhancement of extracellular calcium deposition of the MG63 cells on the treated scaffolds. In summary, the scaffolds S7 (C/H/MFC/Zr) treated with dimethyl glutamate revealed favorable effects on bone tissues, implying a potential towards the treatment of bone defects and drug delivery.