Supplementation with probiotics co-cultivation improves the reproductive performance in a sow-piglet model by mother-infant microbiota transmission and placental mTOR signaling

• Published in: World journal of microbiology & biotechnology 2025, Vol.41 (1), p.13
• Main Authors: Li, Suchen, Lu, Tingting, Lin, Zhixin, Zhang, Yuanyuan, Zhou, Xinchen, Li, Meng, Miao, Hui, Yang, Zhiren, Han, Xinyan
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animals; Animals, Newborn; Applied Microbiology; Bacillus subtilis - physiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Birth Weight; Colostrum; Colostrum - microbiology; Cultivation; Diarrhea; Diarrhea - microbiology; Diet; Dietary Supplements; Environmental Engineering/Biotechnology; Female; Fetuses; Gestation; Hogs; Immunoglobulins; Infants; Lactation; Lactobacillus acidophilus - growth & development; Lactobacillus acidophilus - physiology; Life Sciences; Maternal Nutritional Physiological Phenomena; Microbiology; Microbiota; Microorganisms; Mother-offspring interactions; Offspring; Placenta; Pregnancy; Probiotics; Probiotics - administration & dosage; Reproduction; Signal Transduction; Swine; TOR protein; TOR Serine-Threonine Kinases - metabolism; Vertical transmission (disease); Weaning
• ISSN: 0959-3993; 1573-0972; 1573-0972
• DOI: 10.1007/s11274-024-04222-5

Maternal nutritional supplementation has a profound effect on the growth and development of offspring. FAM ® is produced by co-cultivation of Lactobacillus acidophilus and Bacillus subtilis and has been demonstrated to potentially alleviate diarrhea, improve growth performance and the intestinal barrier integrity of weaned piglets. This study aimed to explore how maternal FAM improves the reproductive performance through mother-infant microbiota, colostrum and placenta. A total of 40 pregnant sows (Landrace × Large White) on d 85 of gestation with a similar parity were randomly divided into two groups ( n  = 20): the control group (Con, basal diet) and the FAM group (FAM, basal diet supplemented with 0.2% FAM). The experimental period was from d 85 of gestation to d 21 of lactation. The results revealed that maternal supplementation with FAM significantly decreased the number of weak-born litters and the incidence of diarrhea, as well as increasing birth weight and average weaning weight, accompanied by increased levels of colostrum nutrient composition and immunoglobulins. In addition, FAM modulated the structure of mother-infant microbiota and promoted the vertical transmission of beneficial bacteria, such as Verrucomicrobiota and Akkermansia . Furthermore, FAM contributed to improving the expression of GLU and AA transporters in the placenta, and increasing the activity of the mTOR signaling pathway. Collectively, maternal supplementation with FAM during late pregnancy and lactation could improve reproductive performance through the transmission of beneficial mother-infant microbiota and placental mTOR signaling pathway and promote fetal development. Graphical abstract