Quercetin preserves mitochondria-endoplasmic reticulum contact sites improving mitochondrial dynamics in aged myocardial cells

• Published in: Biogerontology (Dordrecht) 2024-12, Vol.26 (1), p.29
• Main Authors: Jiménez, Ray, Zúñiga-Muñoz, Alejandra, Álvarez-León, Edith, García-Niño, Wylly Ramsés, Navarrete-Anastasio, Gabriela, Soria-Castro, Elizabeth, Pérez-Torres, Israel, Lira-Silva, Elizabeth, Pavón, Natalia, Cruz-Gregorio, Alfredo, López-Marure, Rebeca, Zazueta, Cecilia, Silva-Palacios, Alejandro
• Format: Article
• Language: English
• Subjects: Aging; Animals; Antioxidants - pharmacology; Biomedical and Life Sciences; Cardiomyocytes; Cardiovascular diseases; Cell Biology; Cell Line; Cellular Senescence - drug effects; D-Galactose; Developmental Biology; Endoplasmic reticulum; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - metabolism; Galactose; Geriatrics/Gerontology; Heart diseases; Life Sciences; Membrane potential; Membrane Potential, Mitochondrial - drug effects; Mitochondria; Mitochondria Associated Membranes; Mitochondria, Heart - drug effects; Mitochondria, Heart - metabolism; Mitochondrial Dynamics - drug effects; Morphology; Muscle contraction; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; Myocytes, Cardiac - pathology; Organelles; Oxidative stress; Oxidative Stress - drug effects; Phenotypes; Quercetin; Quercetin - pharmacology; Rats; Reactive Oxygen Species - metabolism; Research Article; Senescence
• ISSN: 1389-5729; 1573-6768; 1573-6768
• DOI: 10.1007/s10522-024-10174-y

Cardiomyocyte senescence plays a crucial role in the pathophysiology of age-related cardiovascular disease. Senescent cells with impaired contractility, mitochondrial dysfunction, and hypertrophic growth accumulate in the heart during aging, contributing to cardiac dysfunction and remodeling. Mitochondrial dynamics is altered in aging cells, leading to changes in their function and morphology. Such rearrangements can affect the spatially restricted region of the mitochondrial membrane that interacts with reticulum membrane fragments, termed mitochondria-endoplasmic reticulum (ER) contact sites (MERCs). Besides, oxidative stress associated with inefficient organelle turnover can drive cellular senescence. Therefore, in this study, we evaluated the possible association between the senolytic effect of the antioxidant quercetin (Q) and MERCs preservation in a D-galactose-induced cellular senescence model. We found that Q ameliorates the senescent phenotype of H9c2 cells in association with increased mitochondria-ER colocalization, reduced distance between both organelles, and lower ROS production. Moreover, regulation of fusion and fission processes was related with increased mitochondrial ATP production and enhanced transmembrane potential. Overall, our data provide evidence that the inhibitory effect of Q on cellular senescence is associated with preserved MERCs and improved mitochondrial function and morphology, which might contribute to the attenuation of cardiac dysfunction.