Gut microbiota of cattle and horses and their use in the production of ethanol and lactic acid from timothy hay

The gut of herbivorous animals is an untapped source of the microbial consortium that could be used to improve the hydrolysis of lignocellulosic biomass. This study investigated the hydrolysis of Timothy hay by the anaerobic incubation with fecal inocula from four cows (CB) and four horses (HB) for...

Full description

Saved in:
Bibliographic Details
Published in:Biofuels (London) 2024-03, Vol.15 (3), p.291-301
Main Authors: Emara Rabee, Alaa, Lamara, Mebarek, Ishaq, Suzanne L.
Format: Article
Language:English
Subjects:
Bacteroides
biofuels
biomass
carbohydrate metabolism
cattle
cellulase and xylanase
cows
digestive system
endo-1,4-beta-glucanase
Enterococcus
ethanol
feces
glucose
Gut bacteria
hay
herbivores
horses
hydrolysis
inoculum
intestinal microorganisms
lactic acid
Lactococcus
lignocellulose
neutral detergent fiber
prediction
Prevotella
Proteobacteria
Streptococcus
timothy
Weissella
xylanases
xylose
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The gut of herbivorous animals is an untapped source of the microbial consortium that could be used to improve the hydrolysis of lignocellulosic biomass. This study investigated the hydrolysis of Timothy hay by the anaerobic incubation with fecal inocula from four cows (CB) and four horses (HB) for 72 h. The microbial communities colonized timothy hay were analyzed using Illumina Mi-Seq sequencing of 16S rDNA genes. The source of the inoculum affected the structure of microbial communities that were dominated by phylum Firmicutes, Bacteroidetes, and Proteobacteria, and the dominant genera were Enterococcus, Streptococcus, Escherichia-Shigella, Bacteroides, and Prevotella. Moreover, several bacteria were observed exclusively in a specific group. For example, group CB had phylum Fibrobacterota and Spirochaetota besides genera Prevotellaceae YAB2003 and Bacteroides; and group HB had genera Weissella and Lactococcus. Moreover, function prediction revealed that the CB group has a higher abundance of genes related to carbohydrate metabolism. Group CB showed higher neutral detergent fiber degradability (NDFD), gas production, cellulase, xylanase, and xylose, and group HB showed higher dry matter degradability (DMD), glucose, ethanol, and lactic acid. The feces of cattle and horses represent a source of the bacterial consortium for enzyme production and the hydrolysis of lignocellulosic biomass.
ISSN:1759-7269
1759-7277
1759-7277
DOI:10.1080/17597269.2023.2238381