Nitrogen fertilization practices alter microbial communities driven by clonal integration in Moso bamboo

• Published in: The Science of the total environment 2024-05, Vol.924, p.171581-171581, Article 171581
• Main Authors: Cao, Tingting, Shi, Man, Zhang, Junbo, Ji, Hangxiang, Wang, Xiao, Sun, Jilei, Chen, Zhenxiong, Li, Quan, Song, Xinzhang
• Format: Article
• Language: English
• Subjects: bacterial communities; Clonal plants; clones; community structure; denitrification; environment; Fertilization; forest management; microbial biomass; Microbiota; nitrification; Nitrogen; Nitrogen fertilizer; Phyllostachys edulis; Poaceae; progeny; Resource integration; Rhizome; rhizomes; rhizosphere; Rhizosphere processes; Soil; Soil Microbiology; Structure and function of microbial communities
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.171581

Nitrogen (N) fertilization is crucial for maintaining plant productivity. Clonal plants can share resources between connected ramets through clonal integration influencing microbial communities and regulating soil biogeochemical cycling, especially in the rhizosphere. However, the effect of various N fertilization practices on microbial communities in the rhizosphere of clonal ramets remain unknown. In this study, clonal fragments of Moso bamboo (Phyllostachys edulis), consisting of a parent ramet, an offspring ramet, and an interconnecting rhizome, were established in the field. NH4NO3 solution was applied to the parent, offspring ramets or rhizomes to investigate the effect of fertilization practices on the structure and function of rhizosphere microbial communities. The differences in N availability, microbial biomass and community composition, and abundance of nitrifying genes among rhizosphere soils of ramets gradually decreased during the rapid growth of Moso bamboo, irrespective of fertilization practice. The soil N availability variation, particularly in NO3−, caused by fertilization practices altered the rhizosphere microbial community. Soil N availability and stable microbial biomass N in parent fertilization were the highest, being 9.0 % and 18.7 %, as well as 60.8 % and 90.4 % higher than rhizome and offspring fertilizations, respectively. The microbial network nodes and links in rhizome fertilization were 1.8 and 7.5 times higher than in parent and offspring fertilization, respectively. However, the diversity of bacterial community and abundance of nitrifying and denitrifying genes were the highest in offspring fertilization among three practices, which may be associated with increased N loss. Collectively, the rhizosphere microbial community characteristics depended on fertilization practices by altering the clonal integration of N in Moso bamboo. Parent and rhizome fertilization were favorable for N retention in plant-soil system and resulted in more stable microbial functions than offspring fertilization. Our findings provide new insights into precision fertilization for the sustainable Moso bamboo forest management. [Display omitted] •Microbial communities of clonal ramets were similar in the late growth stages.•Parent fertilization showed the highest soil N availability and microbial N levels.•Bacterial interactions were the most complex in rhizome fertilization.•Offspring fertilization raised N loss by enhanced nitrification and denitrif