Inhibition of pyruvate dehydrogenase accelerates anaerobic glycolysis under postmortem simulating conditions

This research aimed to explore the potential influence of mitochondria on the rate of anaerobic glycolysis. We hypothesized that mitochondria could reduce the rate of anaerobic glycolysis and pH decline by metabolizing a portion of glycolytic pyruvate. We utilized an in vitro model and incorporated...

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Bibliographic Details
Published in:Meat science 2024-07, Vol.213, p.109510-109510, Article 109510
Main Authors: Taylor, Mackenzie J., Stafford, Chandler D., Buhler, Jared F., Dang, David S., Alruzzi, Mohammed A., Najm, Teif A., Gerrard, Samuel D., Thornton, Kara J., van Vliet, Stephan, El-Kadi, Samer W., Gerrard, David E., Matarneh, Sulaiman K.
Format: Article
Language:English
Subjects:
acidification
avidin
carbon
glucose
glycogen
glycogenolysis
Glycolysis
Isotope tracer
lactic acid
meat science
metabolites
Mitochondria
phosphofructokinases
phosphorylase
postmortem changes
pyruvate carboxylase
Pyruvate dehydrogenase
pyruvate dehydrogenase (lipoamide)
pyruvate kinase
pyruvic acid
TCA
tricarboxylic acid cycle
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Summary:This research aimed to explore the potential influence of mitochondria on the rate of anaerobic glycolysis. We hypothesized that mitochondria could reduce the rate of anaerobic glycolysis and pH decline by metabolizing a portion of glycolytic pyruvate. We utilized an in vitro model and incorporated CPI-613 and Avidin to inhibit pyruvate dehydrogenase (PDH) and pyruvate carboxylase (PC), respectively. Four treatments were tested: 400 μM CPI-613, 1.5 U/ml Avidin, 400 μM CPI-613 + 1.5 U/ml Avidin, or control. Glycolytic metabolites and pH of the in vitro model were evaluated throughout a 1440-min incubation period. CPI-613-containing treatments, with or without Avidin, decreased pH levels and increased glycogen degradation and lactate accumulation compared to the control and Avidin treatments (P 
ISSN:0309-1740
1873-4138
DOI:10.1016/j.meatsci.2024.109510