Cellular toxicity and DNA damage induced by Newbouldia laevis used for male infertility treatment in prokaryotic and eukaryotic models

• Published in: Protoplasma 2024, Vol.261 (1), p.53-64
• Main Authors: Alabi, Okunola Adenrele, Okorie, Benson, Simon-Oke, Iyabo A., Atanda, Halimat Chisom, Olumurewa, John A. V., Adebo, Taiwo Cosmas
• Format: Article
• Language: English
• Subjects: Allium cepa; Ames test; Biomedical and Life Sciences; Cell Biology; Cell division; childbirth; Chromosome aberrations; Complementation; Cytotoxicity; DNA; DNA damage; Escherichia coli; Genotoxicity; hydroxymethylfurfural; Infertility; Leaves; Life Sciences; male sterility; Mutagenicity; mutagens; Newbouldia laevis; onions; Original Article; phytochemicals; Plant extracts; Plant Sciences; root growth; Salmonella; Salmonella Typhimurium; tap water; VOCs; Volatile organic compounds; Zoology
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-023-01880-4

Leaves of Newbouldia laevis have been extensively used in solving problems associated with infertility and childbirth in many African countries. Yet, information is very limited on the DNA damaging potential of this plant. This study evaluated the cytogenotoxic effect of the aqueous extract of N. laevis leaf using prokaryotic models (Ames Salmonella fluctuation test using TA100 and TA98 strains of Salmonella typhimurium and SOS Chromotest with Escherichia coli PQ37) and eukaryotic model ( Allium cepa root cells). Identification of the volatile organic compounds (VOCs) and phytochemical screening of the plant extract were also performed. Onion bulbs were grown on each concentration (1 to 50%; v/v, extract/tap water) of the extract for chromosomal aberrations and root growth analyses. Results of the Ames test indicated that the extract is mutagenic while the SOS Chromotest results showed good complementation to the Ames test results, although the E. coli PQ37 system showed slightly higher sensitivity in the detection of mutagenicity and genotoxicity of the extract. The plant extract was cytotoxic when compared to the control, inducing a significant ( p < 0.05) concentration-dependent inhibition of root growth from 5 to 50% concentrations. At 50% concentration, the extract completely inhibited cell division in the A. cepa . Also, chromosomal aberration increased significantly ( p < 0.05) in exposed onions from 5 to 20% concentrations. The mutagenicity and cytogenotoxicity recorded in this report were believed to be caused by the presence of VOCs such as 1,2,3-benzene-triol, 1,2-benzenediol, and 5-hydroxymethylfurfural, and alkaloids in the extract an indication of the cytogenotoxicity of the aqueous extract of N. laevis leaf even at low concentration.