A blood-based multi-biomarker approach reveals different physiological responses of common kestrels to contrasting environments

• Published in: Environmental research 2024-06, Vol.251 (Pt 2), p.118674, Article 118674
• Main Authors: Giovanetti, Laura, Caliani, Ilaria, Damiani, Gianluca, Dell’Omo, Giacomo, Costantini, David, Casini, Silvia
• Format: Article
• Language: English
• Subjects: antioxidant activity; antioxidants; Biomarkers; cholinesterase; Common kestrels; complement; DNA damage; Falco tinnunculus; Falconiformes; genotoxicity; glutathione; Italy; landscapes; monocytes; nest boxes; neurotoxicity; Oxidative status; Physiological responses; physiological state; respiratory burst; rural areas; Urbanization
• ISSN: 0013-9351; 1096-0953; 1096-0953
• DOI: 10.1016/j.envres.2024.118674

The increase of urbanization and agricultural activities is causing a dramatic reduction of natural environments. As a consequence, animals need to physiologically adjust to these novel environments, in order to exploit them for foraging and breeding. The aim of this work was to compare the physiological status among nestling common kestrels (Falco tinnunculus) that were raised in nest-boxes located in more natural, rural, or urban areas in a landscape with a mosaic of land uses around Rome in Central Italy. A blood-based multi-biomarker approach was applied to evaluate physiological responses at multiple levels, including antioxidant concentrations, immunological functions, genotoxicity, and neurotoxicity. We found lower concentrations of glutathione and GSH:GSSG ratio values and higher proportions of monocytes in urban birds compared to the other areas. We also found higher DNA damage in rural compared to urban and natural krestels and inhibition of butyrylcholinesterase activity in urban and natural birds compared to rural area. Finally, we found similar values among study areas for respiratory burst, complement system, bactericidal capacity, and plasma non-enzymatic antioxidant capacity. These results suggest that (i) city life does not necessarily cause physiological alterations in kestrels compared to life in other habitats, and (ii) environmental pressures are likely to differ in typology and intensity across habitats requiring specific responses that a multi-biomarker approach can help to detect. Further studies are needed to assess which factors are responsible for the physiological differences among city, rural, and natural birds, and whether these differences are consistent across time and space. [Display omitted] •Urbanization and agricultural activities cause profound environmental changes.•Animals need to adjust their physiology in response to novel environments.•Physiological responses can be investigated by multi-biomarker approach.•Kestrels showed different physiological responses to contrasting environments.•City life was not more stressful than other environments in our study area.