Comparative Analysis of Transcriptome at Different Growth and Development Stages of Camellia oleifera

• Published in: Russian journal of plant physiology 2024-06, Vol.71 (3), p.80-80, Article 80
• Main Authors: Hu, J. J., Song, H., Cao, Z. H., Shu, Q. L., Han, B. X.
• Format: Article
• Language: English
• Subjects: Biodegradation; Biological activity; Biomedical and Life Sciences; Biosynthesis; Camellia oleifera; Carbohydrate metabolism; Carbohydrates; Comparative analysis; Cycles; Degradation; Developmental stages; Energy metabolism; Enrichment; Environmental degradation; Flowering; Flowers; Fruits; Gene expression; gene expression regulation; gene ontology; Gene sequencing; Genes; hypocotyls; juveniles; Leaves; LHY gene; Life Sciences; loci; Metabolic pathways; Metabolism; Plant Physiology; Plant Sciences; Plasma membranes; Research Papers; ribosomes; Sequence analysis; transcriptome; Transcriptomes
• ISSN: 1021-4437; 1608-3407
• DOI: 10.1134/S1021443724604488

To investigate the gene expression and metabolic pathways involved in flower development at different growth stages of Camellia oleifera , this study focused on leaves collected during the juvenile stage, initial fruiting stage, and peak fruiting stage of the ‘Dabieshan 1’ C. oleifera variety. Transcriptome sequencing analysis was performed to obtain the transcriptome data of leaf samples during the pre-flowering period. The annotated genes included key regulators of flower development pathways, such as FLOWERING LOCUS C ( FLC) , SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 ( SOC1) , SUPPRESSOR OVEREXPRESSION CONSTANS 2 ( SOC2) , as well as genes involved in the photoperiod pathway, including CYCLING DOF FACTOR 2 ( CDF2) , CYCLING DOF FACTOR 3 ( CDF3) , and LATE ELONGATED HYPOCOTYL ( LHY) . Specifically, 7 UniGene sequences of the CDF2 gene, 3 UniGene sequences of the CDF3 gene, and 3 UniGene sequences of the LHY gene were identified, highlighting their important roles in flower development regulation in C. oleifera . Gene Ontology analysis demonstrated enrichment in various biological processes related to carbohydrate metabolism, biosynthesis, and environmental response, while differentially expressed genes in the molecular function category were associated with degradation activities. In terms of cellular components, the differentially expressed genes were enriched in ribosome and plasma membranes. Kyoto Encyclopedia of Genes and Genomes analysis revealed enrichment in multiple metabolic pathways, biosynthesis, and degradation across the three developmental stages of C. oleifera leaves. These findings provide a foundation for further investigation into the energy metabolism involved in the regulation of flower development in C. oleifera .