Development of polymorphic microsatellite markers for genetic stock identification of green chromide, Etroplus suratensis using next generation sequencing technology

Thirty polymorphic microsatellites were developed and characterised for the aquaculture important species, Green chromide, Etroplus suratensis through Illumina Sequencing with a mean read quality (Phred score) of 35.8 and the microsatellite primers were designed from the sequenced information, consi...

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Bibliographic Details
Published in:Conservation genetics resources 2024-06, Vol.16 (2), p.191-194
Main Authors: Christo, Magdeline, Jose, Divya Merin, Divya, P. R., Rekha, M. U., Sarkar, U. K.
Format: Article
Language:English
Subjects:
alleles
Animal Genetics and Genomics
aquaculture
Biodiversity
Biomedical and Life Sciences
computer software
Conservation Biology/Ecology
Ecology
Etroplus suratensis
Evolutionary Biology
Fish
Genetic markers
Genetics
Heterozygosity
Life Sciences
Microsatellite Records
microsatellite repeats
Microsatellites
Next-generation sequencing
Plant Genetics and Genomics
species
world wide web
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Summary:Thirty polymorphic microsatellites were developed and characterised for the aquaculture important species, Green chromide, Etroplus suratensis through Illumina Sequencing with a mean read quality (Phred score) of 35.8 and the microsatellite primers were designed from the sequenced information, considering various attributes such as the number of repeats, Tm, GC content, Product size, etc. using the software PRIMER 3.0 web tool. The allele numbers ranged from 6 to 18. The mean observed heterozygosity was 0.745 and the mean expected heterozygosity was 0.762. The average PIC value for 30 microsatellite primers was 0.80 and all the loci had PIC values > 0.75. The identified polymorphic loci would help in revealing the genetic stock identification among the Pearlspot population.
ISSN:1877-7260
1877-7252
1877-7260
DOI:10.1007/s12686-024-01350-w