Acoustic-holography-patterned primary hepatocytes possess liver functions

Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and...

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Bibliographic Details
Published in:Biomaterials 2024-12, Vol.311, p.122691, Article 122691
Main Authors: Li, Changcan, Xu, Gang, Wang, Yinhan, Huang, Laixin, Cai, Feiyan, Meng, Long, Jin, Bao, Jiang, Zhuoran, Sun, Hang, Zhao, Haitao, Lu, Xin, Sang, Xingting, Huang, Pengyu, Li, Fei, Yang, Huayu, Mao, Yilei, Zheng, Hairong
Format: Article
Language:English
Subjects:
3D model
Acoustic holography
Acoustics
Animals
biocompatible materials
Cells, Cultured
drugs
hepatocytes
Hepatocytes - cytology
Hepatocytes - metabolism
holography
Holography - methods
liver
Liver - cytology
Liver function
Mice
Mice, Inbred C57BL
pathogenesis
Primary hepatocytes
Spheroids, Cellular - cytology
therapeutics
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Summary:Acoustic holography (AH), a promising approach for cell patterning, emerges as a powerful tool for constructing novel invitro 3D models that mimic organs and cancers features. However, understanding changes in cell function post-AH remains limited. Furthermore, replicating complex physiological and pathological processes solely with cell lines proves challenging. Here, we employed acoustical holographic lattice to assemble primary hepatocytes directly isolated from mice into a cell cluster matrix to construct a liver-shaped tissue sample. For the first time, we evaluated the liver functions of AH-patterned primary hepatocytes. The patterned model exhibited large numbers of self-assembled spheroids and superior multifarious core hepatocyte functions compared to cells in 2D and traditional 3D culture models. AH offers a robust protocol for long-term in vitro culture of primary cells, underscoring its potential for future applications in disease pathogenesis research, drug testing, and organ replacement therapy.
ISSN:0142-9612
1878-5905
1878-5905
DOI:10.1016/j.biomaterials.2024.122691