Molecular phylogenetic reconstruction improves the taxonomic understanding of Indian Dipcadi (Asparagaceae) and reveals a new species from the bank of Hiranyakeshi River, Maharashtra, India

• Published in: Journal of plant research 2024-09, Vol.137 (5), p.829-845
• Main Authors: Shelke, P. E., Tamboli, A. S., Surveswaran, S., Yadav, S. R., Choo, Y.-S., Pak, J. H., Lekhak, M. M.
• Format: Article
• Language: English
• Subjects: Asparagaceae; Asparagaceae - classification; Asparagaceae - genetics; Biogeography; Biomedical and Life Sciences; cohesion; data collection; DNA, Plant - genetics; Egypt; flower tepals; genus; India; Indigenous species; landscapes; Life Sciences; monophyly; New species; Nucleotides; Ornithogaloideae; Ovules; Phylogenetics; Phylogeny; Plant Biochemistry; Plant Ecology; Plant Physiology; Plant Sciences; Plastids - genetics; polyphyly; Regular Paper – Taxonomy/Phylogenetics/Evolutionary Biology; rivers; Sequence Analysis, DNA; species; subfamily; Taxa; Taxonomy
• ISSN: 0918-9440; 1618-0860; 1618-0860
• DOI: 10.1007/s10265-024-01558-9

Dipcadi (Scilloideae: Asparagaceae) is a genus of bulbous monocots with approximately 40 species, of which 13 occur in India. Species delimitation within the genus has been troublesome hindering a comprehensive phylogenetic analysis. The most recent phylogeny of the subfamily Ornithogaloideae included six species of Dipcadi only from Africa. Here, we reconstructed the phylogeny of Ornithogaloideae including 23 accessions comprising 13 recognized taxa (11 species and two varieties) of Indian Dipcadi . The phylogenetic analyses were based on nucleotide sequences of three plastid regions ( rbc L, mat K and trn L-F spacer) and one nuclear region (ITS). Pseudogaltonia clavata exhibited sister relationship to Dipcadi . Our combined nuclear + plastid dataset analyses revealed a monophyletic Dipcadi with five clades, Clade I-V. Clade I, II and III included mainly Indian species whereas Clade V included mostly African species. Clade IV comprised D. serotinum . Clade I included nine taxa including our newly described species, D. mukaianum . The new species was phylogenetically placed with D. erythraeum , D. saxorum and D. ursulae . Morphologically, the species resembled D. montanum and D. ursulae but differed in characters such as tepal cohesion, number of ovules per locule and foul-smelling flowers. Clade II and III included 11 and six taxa, respectively. D. erythraeum which has a native range from Egypt to western India was found in Clades I and V. The widespread Dipcadi species, viz. D. erythraeum and D. serotinum showed polyphyly however, the monophyly of Dipcadi is established. Our studies suggest that additional molecular markers (plastid as well as nuclear) should be tested for their taxonomy utility. Further work on the historical biogeography of Dipcadi on the subfamily Ornithogaloideae with more genetic data will yield insights how aridification of the landscape would have shaped the evolution of the geographical clades.