Extracellular vesicles from Staphylococcus aureus promote the pathogenicity of Pseudomonas aeruginosa

• Published in: Microbiological research 2024-04, Vol.281, p.127612-127612, Article 127612
• Main Authors: Subsomwong, Phawinee, Teng, Wei, Ishiai, Takahito, Narita, Kouji, Sukchawalit, Rojana, Nakane, Akio, Asano, Krisana
• Format: Article
• Language: English
• Subjects: Anti-phagocytosis; antibiotics; Biofilm; biosynthesis; dyes; epithelial cells; epithelium; Extracellular vesicles; immune response; lipophilicity; Lipopolysaccharide biosynthesis; lipopolysaccharides; macrophages; pathogenicity; protein transport; proteomics; Pseudomonas aeruginosa; Staphylococcus aureus; synergism
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2024.127612

Co-infections with Staphylococcus aureus and Pseudomonas aeruginosa are common in patients with chronic wounds, but little is known about their synergistic effect mediated by extracellular vesicles (EVs). In this study, we investigated the effect of EVs derived from S. aureus (SaEVs) on the pathogenicity of P. aeruginosa. By using lipophilic dye, we could confirm the fusion between SaEV and P. aeruginosa membranes. However, SaEVs did not alter the growth and antibiotic susceptible pattern of P. aeruginosa. Differential proteomic analysis between SaEV-treated and non-treated P. aeruginosa was performed, and the results revealed that lipopolysaccharide (LPS) biosynthesis protein in P. aeruginosa significantly increased after SaEV-treatment. Regarding this result, we also found that SaEVs promoted LPS production, biofilm formation, and expression of polysaccharide polymerization-related genes in P. aeruginosa. Furthermore, invasion of epithelial cells by SaEV-pretreated P. aeruginosa was enhanced. On the other hand, uptake of P. aeruginosa by RAW 264.7 macrophages was impaired after pretreatment P. aeruginosa with SaEVs. Proteomic analysis SaEVs revealed that SaEVs contain the proteins involving in host cell colonization, inhibition of host immune response, anti-phagocytosis of the macrophages, and protein translocation and iron uptake of S. aureus. In conclusion, SaEVs serve as a mediator that promote P. aeruginosa pathogenicity by enhancing LPS biosynthesis, biofilm formation, epithelial cell invasion, and macrophage uptake impairment. [Display omitted] •SaEVs mediate P. aeruginosa pathogenicity.•SaEV-treated P. aeruginosa has a high level of LPS biosynthesis protein.•SaEVs promote the P. aeruginosa biofilm formation.•SaEVs increase the cell invasion of P. aeruginosa to epithelial cells.•SaEVs prevent P. aeruginosa uptake by macrophage cells.