Genome-centric polyhydroxyalkanoate reconciliation reveals nutrient enriched growth dependent biosynthesis in Bacillus cereus IBA1

• Published in: Bioresource technology 2023-08, Vol.382, p.129210-129210, Article 129210
• Main Authors: Kumar, Rajat, Li, Dongyi, Luo, Liwen, Manu, M.K., Zhao, Jun, Tyagi, Rajeshwar D., Wong, Jonathan W.C.
• Format: Article
• Language: English
• Subjects: Bacillus cereus; Bacillus cereus - metabolism; biogenesis; Biopolymer; Biopolymers - metabolism; biosynthesis; endotoxins; Gram-positive; Gram-positive bacteria; industrial applications; Nutrient conditioning; Nutrients; operon; PHA granule associated proteins; polyhydroxyalkanoates; Polyhydroxyalkanoates - metabolism; regulatory proteins; sustainable development; technology; Transcription Factors - metabolism; Whole genome
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2023.129210

[Display omitted] •Environmental isolation of new gram-positive strain i.e., Bacillus cereus IBA1.•Whole genome reconciliation of PHA associated pathways.•Innovative non-stress limiting nutrient enriched fermentation.•Confirmed growth dependent intracellular PHA granular accumulation.•Expression profiling of genes encoding PHA granule associated proteins (PGAPs). Microbiological polyhydroxyalkanoates (PHAs) are rooted as the most promising bio-replacements of synthetic polymers. Inherent properties of these PHAs further expand their applicability in numerous industrial, environmental, and clinical sectors. To propel these, a new environmental, endotoxin free gram-positive bacterium i.e., Bacillus cereus IBA1 was identified to harbor advantageous PHA producer characteristics through high-throughput omics mining approaches. Unlike traditional fermentations, nutrient enriched strategy was used to enhance PHA granular concentrations by ∼2.3 folds to 2.78 ± 0.19 g/L. Additionally, this study is the first to confirm an underlying growth dependent PHA biogenesis through exploring PHA granule associated operons which harbour constitutively expressing PHA synthase (phaC) coupled with differentially expressing PHA synthase subunit (phaR) and regulatory protein (phaP, phaQ) amid different growth phases. Moreover, the feasibility of this promising microbial phenomenon could propel next-generation biopolymers, and increase industrial applicability of PHAs, thereby significantly contributing to the sustainable development.