Characterization of Shiga toxin-producing Escherichia coli presence, serogroups and risk factors from private groundwater sources in western Ireland

• Published in: The Science of the total environment 2023-03, Vol.866, p.161302, Article 161302
• Main Authors: Burke, Liam Patrick, Chique, Carlos, Fitzhenry, Kelly, Chueiri, Alexandra, O'Connor, Louise, Hooban, Brigid, Cahill, Niamh, Brosnan, Ellen, Olaore, Lateefat, Sullivan, Emma, Reilly, Louise, Morris, Dearbháile, Hynds, Paul, O'Dwyer, Jean
• Format: Article
• Language: English
• Subjects: Animals; autumn; DNA; E. coli; enteritis; environment; Escherichia coli O104; Escherichia coli Proteins - genetics; Feces; genes; Groundwater; groundwater contamination; Ireland; Ireland - epidemiology; Private wells; quantitative polymerase chain reaction; rain; risk; Risk Factors; septic systems; Serogroup; serotypes; Sheep; Shiga toxin; Shiga-Toxigenic Escherichia coli; STEC
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.161302

Over recent years, Ireland has reported the highest crude incidence rates of Shiga toxin-producing Escherichia coli (STEC) enteritis in Europe. Unregulated private groundwater sources have emerged as an important potential transmission route for STEC, with up to 750,000 Irish residents reliant on these sources for domestic waters. This study aimed to investigate the prevalence and serogroup profile of STEC contamination from domestic private wells in western Ireland. Fifty-two groundwater sources were analysed during two sampling campaigns in the autumn (September/October) of 2019 (n = 21) and 2021 (n = 31). Untreated groundwater samples (30 L) were collected and analysed using the “CapE” (capture, amplify, extract) method. Extracted DNA was tested using multiplex real-time PCR for Shiga toxin stx1 and/or stx2 and eae genes. STEC positive DNA samples were tested for clinically relevant serogroups by real-time PCR. Data relating to 27 potential groundwater contamination risk factors were geospatially linked to each well and assessed for association with E. coli, stx1 and/or stx2 and eae presence/absence. Overall, 20/52 wells (38.4 %) were positive for E. coli (median concentration 8.5 MPN/100 mL as assessed by Colilert-18 method). Stx1 and/or stx2 was detected in 10/52 (19.2 %) wells overall and 8/20 E. coli positive wells, equating to a STEC to “generic” E. coli detection ratio of 40 %. Six of these wells (30 %) were also positive for eae. One or more serogroup-specific gene targets were identified in all but one stx1 and/or stx2 positive sample, with O145 (n = 6), O157 (n = 5) and O103 (n = 4) most prevalent. STEC presence was significantly associated with decreasing well depth (U = −2.243; p = 0.024) and increasing 30-day mean antecedent rainfall (U = 2.126; p = 0.034). Serogroup O104 was associated with increased sheep density (U = 2.089; p = 0.044) and detection of stx1 and/or stx2 + eae with increased septic tank density (U = 2.246 p = 0.023). Findings indicate high detection rates of clinically relevant STEC in E. coli contaminated groundwater sources in Ireland. [Display omitted] •Field studies reveal high STEC contamination rate in wells positive for E. coli.•Multiple clinically relevant STEC serogroups detected in wells with high E. coli counts.•High antecedent rainfall, shallow well depth and septic tank density associated risk factors.