Preparation of Yeast Extract from Brewer’s Yeast Waste and Its Potential Application as a Medium Constituent

Yeast extract serves as a source of nutritional components essential for human dietary requirements, feed formulations, and the vital growth factors and nutrients necessary for microorganisms. However, the production cost of yeast extract using cultivated active dry yeast is relatively high. This st...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2024-10, Vol.196 (10), p.6608-6623
Main Authors: Gao, Feng, Li, Qingfei, Wei, Wei, Wang, Yutao, Song, Wancheng, Yang, Xurui, Ji, Huai, Zhou, Jia, Xin, Ya, Tan, Zhongbiao, Pei, Jianjun, Shi, Hao
Format: Article
Language:English
Subjects:
amino nitrogen
Autolysis
Beer
Beer - microbiology
beers
Biochemistry
Biotechnology
Breweries
Chemistry
Chemistry and Materials Science
Comparative analysis
Culture Media - chemistry
Enzymes
Growth factors
humans
Hydrogen-Ion Concentration
Hydrolysis
microbial growth
Microorganisms
Nutrients
Nutritional requirements
Original Article
Production costs
Reclamation
Saccharomyces cerevisiae - metabolism
Temperature
Temperature requirements
wastes
Yeast
yeast extract
Yeasts
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Summary:Yeast extract serves as a source of nutritional components essential for human dietary requirements, feed formulations, and the vital growth factors and nutrients necessary for microorganisms. However, the production cost of yeast extract using cultivated active dry yeast is relatively high. This study aims to utilize the autolysis of discarded yeast post beer brewing to produce yeast extract. The concentration, temperature, pH, and time conditions are systematically optimized. It reveals that the yield of amino nitrogen and solids in the extract was increased by 3.3% and 20.9% under the optimized conditions (1.2% wall-breaking enzyme, 1% yeast extract enzyme, and a hydrolysis time of 24 h) than that of the documented 4.03% and 69.05%. Additionally, a comparative analysis with commercially available yeast powder demonstrates that the yeast extract derived from this study adequately fulfills the nutritional requirements for microbial growth. Hence, the utilization of discarded beer yeast presents an opportunity for the valuable reclamation of waste yeast, showcasing promising potential applications.
ISSN:0273-2289
1559-0291
1559-0291
DOI:10.1007/s12010-024-04885-8