Biotransformation and oxidative stress markers in yellowfin seabream (Acanthopagrus latus): Interactive impacts of microplastics and florfenicol

• Published in: The Science of the total environment 2024-12, Vol.954, p.176661, Article 176661
• Main Authors: Shirmohammadi, Mehrnaz, Kianersi, Farahnaz, Shiry, Nima, Burgos-Aceves, Mario Alberto, Faggio, Caterina
• Format: Article
• Language: English
• Subjects: Acanthopagrus; Acanthopagrus latus; Animals; Antioxidant system; biomarkers; Biomarkers - metabolism; Biotransformation; catalase; Catalase - metabolism; environment; fish; Florfenicol; glutathione peroxidase; Glutathione Peroxidase - metabolism; glutathione transferase; Glutathione Transferase - metabolism; kidneys; liver; Liver - drug effects; Liver - metabolism; malondialdehyde; Malondialdehyde - metabolism; Microplastics; Microplastics - toxicity; Oxidative damage; oxidative stress; Oxidative Stress - drug effects; Sea Bream - metabolism; superoxide dismutase; Superoxide Dismutase - metabolism; Thiamphenicol - analogs & derivatives; Thiamphenicol - toxicity; toxicity; Water Pollutants, Chemical - toxicity
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2024.176661

This study investigates the combined toxicity of microplastics (MPs) and florfenicol (FLO) on biotransformation enzymes and oxidative stress biomarkers in the liver and kidney of yellowfin seabream (Acanthopagrus latus). Fish were fed 15 mg kg−1 of FLO and 100 or 500 mg kg−1 of MPs for 10 days. Biomarkers, including ethoxyresorufin-O-deethylase, glutathione-S-transferase, superoxide dismutase, catalase, glutathione peroxidase, malondialdehyde (MDA), and protein carbonylation (PC), were measured in both organs at 1, 7, and 14 days post-exposure. FLO levels peaked on day 1 and declined after that. Liver biomarkers were more responsive to pollutants, with the combined exposure of FLO and MPs leading to more pronounced toxicity. By day 14, only the FLO group showed a return to baseline biomarker levels, while MDA and PC levels remained elevated in MPs and co-exposed groups. These findings highlight the importance of considering the interactive effects of multiple pollutants in addressing marine environmental stressors. [Display omitted] •FLO and MPs altered antioxidant and biotransformation activities in seabream liver/kidney.•MPs, alone and with FLO, caused oxidative damage in seabream liver and kidney.•MPs (50 & 100 ppm) amplified FLO's toxic effects.•MPs increased FLO (15 ppm) bioaccumulation in seabream liver and kidney.•MPs and FLO exposure should follow specific aquatic safety standards.•Combined toxicity of MPs and FLO poses risks to fish health and ecosystem balance.