Lauryl-NrTP6 lipopeptide self-assembled nanorods for nuclear-targeted delivery of doxorubicin

• Published in: Nanoscale 2025-01
• Main Authors: Phungula, Amanda, Zuffi, Sofia, Thongsom, Sunisa, Di Gianvincenzo, Paolo, Reyes, Santiago Gimenez, Caribé Dos Santos Valle, Ana Beatriz, Pittella, Frederico, Albericio, Fernando, de la Torre, Beatriz G, Moya, Sergio E
• Format: Article
• Language: English
• ISSN: 2040-3372; 2040-3372
• DOI: 10.1039/d4nr04068f

Targeted delivery offers solutions for more efficient therapies with fewer side effects. Here, lipopeptides (LPs) prepared by conjugation of the nuclear-targeting peptide analogue H-YKQSHKKGGKKGSG-NH (NrTP6) and two lauric acid chains are used to encapsulate the chemotherapeutic agent doxorubicin (DX) through a solvent-exchange protocol. LPs spontaneously form nanosized rod-like assemblies in phosphate buffer. DX is trapped in the peptide regions of the assemblies. Confocal laser scanning microscopy shows that the peptide assemblies translocate into the nucleus. Cytotoxicity studies over 72 h in A549 and HeLa cancer cell lines show less toxicity for the LP encapsulated DX than for free DX. In contrast, subtoxic doses of encapsulated DX are more effective than free DX in avoiding colony formation over 14 days, with a complete absence of colonies for the LP-encapsulated DX. The results show a more efficient and slow delivery of DX to the nucleus through LP encapsulation, paving the way for the use of lower DX doses as a chemotherapeutic agent.