Less Can Be More: Optimization of the Acquisition Range to Enhance Phasor Analysis in Fluorescence Lifetime Imaging Microscopy

The phasor approach is widely recognized as a powerful method for analyzing multidimensional images, and is currently one of the most utilized algorithms for spectral unmixing in fluorescence microscopy. Notably, it is exstensively employed in fluorescence lifetime imaging microscopy, but limitation...

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Bibliographic Details
Published in:Microscopy and microanalysis 2025-04, Vol.31 (2)
Main Authors: Di Giacinto, Flavio, Serantoni, Cassandra, De Spirito, Marco, Maulucci, Giuseppe
Format: Article
Language:English
Subjects:
Algorithms
Biological samples
Fluorescence
Fluorescence microscopy
Image Processing, Computer-Assisted - methods
Imaging
Lifetime
Microscopy
Microscopy, Fluorescence - methods
Optical Imaging - methods
Optimization
Parameters
Phasors
Online Access:Get full text
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