Dramatic Increase in Stability and Longevity of Enzymes Attached to Monodispersive Iron Nanoparticles

Enzymes are proteins that function as biocatalysts in living systems. One of the major concerns in environmental, biomedical, and other applications of enzymes is their short lifetime (J. Kim, "Single-Enzyme Nanoparticles on Nanostructured Matrices," 2003). Enzymes lose their activity due...

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Bibliographic Details
Published in:IEEE transactions on magnetics 2007-06, Vol.43 (6), p.2418-2420
Main Authors: Sharma, A., You Qiang, Antony, J., Meyer, D., Kornacki, P., Paszczynski, A.
Format: Article
Language:English
Subjects:
Biochemistry
Conjugate
Conjugates
Cross-disciplinary physics: materials science
rheology
Economic forecasting
Economics
environment application
enzyme
Enzymes
Exact sciences and technology
Iron
iron nanoparticle
Laboratories
Longevity
Magnetic cores
Magnetic shielding
Magnetism
Materials science
Nanoparticles
Nanostructure
Other topics in materials science
Oxidation
Physics
Proteins
Stability
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Summary:Enzymes are proteins that function as biocatalysts in living systems. One of the major concerns in environmental, biomedical, and other applications of enzymes is their short lifetime (J. Kim, "Single-Enzyme Nanoparticles on Nanostructured Matrices," 2003). Enzymes lose their activity due to oxidation or other processes which results in less stability and a shorter lifetime thereby rendering them less efficient. An effective way to increase the stability, longevity, and reusability of the enzymes is to attach them to magnetic iron nanoparticles. With this aim, two different catabolic enzymes, trypsin and peroxidase, were attached to uniform magnetic core-shell iron nanoparticles (MNPs) produced in our laboratory. Our study indicates that the lifetime of enzymes increases dramatically from a few hours to weeks and that MNP-Enzyme conjugates are more stable, efficient, and economical. We predict that MNPs shield the enzymes preventing them from getting oxidized or self digested. This results in an increased lifetime of the enzymes. Since the magnetization of MNPs is high (~140 emu/g), MNP-enzyme conjugates could efficiently be reused, which makes enzymes more productive. We also found that the enzyme structure plays a major role in efficient attachment of MNPs.
ISSN:0018-9464
1941-0069
DOI:10.1109/TMAG.2007.893849