Depth profiles of bacterioplankton assemblages and their activities in the Ross Sea

• Published in: Deep-sea research. Part I, Oceanographic research papers Oceanographic research papers, 2009-12, Vol.56 (12), p.2193-2205
• Main Authors: Celussi, Mauro, Cataletto, Bruno, Fonda Umani, Serena, Del Negro, Paola
• Format: Article
• Language: English
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Antarctica; Bacteria; Biogeochemical cycles; Biological and medical sciences; Earth, ocean, space; Exact sciences and technology; External geophysics; Fundamental and applied biological sciences. Psychology; Genotype & phenotype; Marine; Marine biology; Oceanography; Oceans; PCR-DGGE; Physics of the oceans; Ross Sea; Sea water ecosystems; Synecology
• ISSN: 0967-0637; 1879-0119
• DOI: 10.1016/j.dsr.2009.09.001

The identification of bacterial community structure has led, since the beginning of the 1990s, to the idea that bacterioplankton populations are stratified in the water column and that diverse lineages with mostly unknown phenotypes dominate marine microbial communities. The diversity of depth-related assemblages is also reflected in their patterns of activities, as bacteria affiliated to different groups can express different activities in a given ecosystem. We analysed bacterial assemblages (DGGE fingerprinting) and their activities (prokaryotic carbon production, protease, phosphatase, chitinase, beta-glucosidase and lipase activities) in two areas in the Ross Sea, differing mainly in their productivity regime: two stations are located in the Terra Nova Bay polynya area (highly productive during summer) and two close to Cape Adare (low phytoplankton biomass and activity). At every station a pronounced stratification of bacterial assemblages was identified, highlighting epipelagic communities differing substantially from the mesopelagic and the bathypelagic communities. Multivariate analysis suggested that pressure and indirectly light-affected variables (i.e. oxygen and fluorescence) had a great effect on the bacterial communities outcompeting the possible influences of temperature and dissolved organic carbon concentration. Generally activities decreased with depth even though a signal of the Circumpolar Deep Water (CDW) at one of the northern stations corresponded to an increase in some of the degradative activities, generating some ‘hot spots’ in the profile. We also found that similar assemblages express similar metabolic requirements reflected in analogous patterns of activity (similar degradative potential and leucine uptake rate). Furthermore, the presence of eukaryotic chloroplasts’ 16S rDNA in deep samples highlighted how in some cases the dense surface-water formation (in this case High Salinity Shelf Water—HSSW) and downwelling can affect, at least for some phylotypes, the bacterial (16S rDNA based) community structure of the dark ocean.