Reduction in levels of amphiphysin 1 mRNA in the hippocampus of aged rats subjected to repeated variable stress

Various neurobiological studies of aging indicate that elevated levels of circulating glucocorticoids lead to hippocampal vulnerability to stress, though little is known about the molecular mechanism underlying stress vulnerability in the elderly. We have compared the gene expression profiles in the...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience 2004, Vol.126 (2), p.461-466
Main Authors: Sawada, T, Morinobu, S, Tsuji, S, Kawano, K, Watanabe, T, Suenaga, T, Takahashi, T, Yamawaki, S, Nishida, A
Format: Article
Language:English
Subjects:
aging
Aging - genetics
Aging - metabolism
amphiphysin 2
Animals
Biological and medical sciences
cDNA array
Fundamental and applied biological sciences. Psychology
G1/S-specific cyclin E
Gene Expression Profiling - methods
Hippocampus - metabolism
learning and memory
Male
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - genetics
Rats
Rats, Sprague-Dawley
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
stress vulnerability
Stress, Physiological - genetics
Stress, Physiological - metabolism
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Various neurobiological studies of aging indicate that elevated levels of circulating glucocorticoids lead to hippocampal vulnerability to stress, though little is known about the molecular mechanism underlying stress vulnerability in the elderly. We have compared the gene expression profiles in the hippocampus of aged (20 months) and adult (3 months) rats in response to repeated variable stress (RVS) for 4 days, using a cDNA array technique and real-time quantitative PCR, to identify putative genes involved in the mechanism of stress vulnerability in the elderly. We found a significant decrease in the levels of amphiphysin 1 mRNA in aged rats subjected to RVS compared with treated and untreated adult rats or to untreated aged rats. Similarly, we found a significant decrease in hippocampal levels of amphiphysin 1 mRNA in aged rats subjected to RVS for 8 days, but not in those subjected to a single VS. These findings suggest that the decrease in the hippocampal levels of amphiphysin 1 mRNA in response to repeated stress may be involved in the stress vulnerability in the elderly, and may lead to the disturbance of learning and memory under stressful conditions in the elderly.
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2004.03.001