Testicular cytochrome P450scc and LHR as possible targets of 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) in the mouse

• Published in: Molecular and cellular endocrinology 2004-06, Vol.221 (1), p.87-96
• Main Authors: Fukuzawa, Noriho H, Ohsako, Seiichiroh, Wu, Qing, Sakaue, Motoharu, Fujii-Kuriyama, Yoshiaki, Baba, Tadashi, Tohyama, Chiharu
• Format: Article
• Language: English
• Subjects: Adrenal Glands - chemistry; Adrenal Glands - metabolism; AhR-null mouse; Animals; Cholesterol Side-Chain Cleavage Enzyme - genetics; Cholesterol Side-Chain Cleavage Enzyme - metabolism; Estradiol; Estradiol - analogs & derivatives; Estradiol - pharmacology; Follicle Stimulating Hormone, beta Subunit - genetics; Follicle Stimulating Hormone, beta Subunit - metabolism; Glycoprotein Hormones, alpha Subunit - genetics; Glycoprotein Hormones, alpha Subunit - metabolism; Intratesticular testosterone; LHR; Luteinizing Hormone, beta Subunit - genetics; Luteinizing Hormone, beta Subunit - metabolism; Male; Mice; Mice, Mutant Strains; Mutation - genetics; P450scc; Pituitary Gland - chemistry; Pituitary Gland - metabolism; Polychlorinated Dibenzodioxins - pharmacology; Receptors, Aryl Hydrocarbon - genetics; Receptors, Aryl Hydrocarbon - physiology; Receptors, LH - genetics; Receptors, LH - metabolism; Receptors, LHRH - genetics; Receptors, LHRH - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; TCDD; Testis - chemistry; Testis - drug effects; Testis - metabolism; Testosterone - analysis; Testosterone - metabolism
• ISSN: 0303-7207; 1872-8057
• DOI: 10.1016/j.mce.2004.02.005

Exposure to 2,3,7,8-tetrachlorodibenzo- p-dioxin (TCDD) in adult animals has been reported to perturb the regulation of steroidogenesis in the testis, possibly by arylhydrocarbon receptor (AhR). To clarify how AhR is involved in the testicular steroidogenesis, we carried out comparative experiments using wild-type and AhR-null male mice that were intraperitoneally administered TCDD. The TCDD administration to wild-type mice showed significant reduction of P450scc and LHR in the testis, whereas the levels in the AhR-null mouse testis were unchanged. To compare anti-androgenic properties on hypothalamo–pituitary–gonadal (HPG) axis, estradiol-3-benzoate (EB), a synthetic estrogen agonist, was administered to mice, the expression of the LHα/ FSHα, LHβ, FSHβ and GnRHR genes was severely impaired in the pituitary gland, in contrast to no observed effects in the TCDD-treated mice. In addition, the expression of the LHR gene was increased in the testis of the EB-treated mice. These observations suggest that the target of TCDD is different from that of EB on HPG axis and that TCDD treatment suppresses the P450scc and LHR genes in the testis in an AhR-dependent manner.