The nonresponse to hepatitis B vaccination is associated with impaired lymphocyte activation

Nonresponsiveness against hepatitis B vaccination has been described in 4–10% of immunized subjects. We have explored the specific cell response to hepatitis B surface antigen by analyzing: PBMC proliferation, cytokine production (Th1, Th2 profiles, and TGF-β), and activation molecules on Th cells....

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2004-08, Vol.326 (1), p.20-28
Main Authors: Goncalves, Loredana, Albarran, Benibelks, Salmen, Siham, Borges, Lerida, Fields, Howard, Montes, Henry, Soyano, Andres, Diaz, Yuleima, Berrueta, Lisbeth
Format: Article
Language:English
Subjects:
Adult
Antigens, CD - analysis
Antigens, Differentiation, T-Lymphocyte - analysis
CD25
CD40 Ligand - analysis
CD40L
CD69
Cells, Cultured
Cytokines - analysis
Flow Cytometry
HBsAg
HBV vaccine
Hepatitis B - blood
Hepatitis B - immunology
Hepatitis B - prevention & control
Hepatitis B Surface Antigens - immunology
Hepatitis B Vaccines - administration & dosage
Humans
Immunity, Cellular
Lectins, C-Type
Lymphocyte Activation
Middle Aged
Nonresponders
Receptors, Interleukin-2 - analysis
T cell activation
T-Lymphocytes - immunology
Treatment Failure
Vaccination
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Summary:Nonresponsiveness against hepatitis B vaccination has been described in 4–10% of immunized subjects. We have explored the specific cell response to hepatitis B surface antigen by analyzing: PBMC proliferation, cytokine production (Th1, Th2 profiles, and TGF-β), and activation molecules on Th cells. A poor proliferative response was demonstrated in nonresponders ( P < 0.05). T cells from responders produced all tested cytokines ( P < 0.01), in contrast with nonresponders subjects ( P < 0.05). Expression of CD69 and CD25 was diminished in T cells from nonresponders ( P < 0.01). A reduced expression of CD40L was also detected in T cells from nonresponders ( P < 0.01). An elevated correlation coefficient was observed between CD40L on CD4+ cells and antibody production. These results suggest an overall inability of T cells to be activated which could be consistent with potential differences in antigen presentation. In conclusion, our results suggest that an altered Th response may be a consequence of inappropriate early activation events.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2004.04.042