Bovine serum albumin conformational changes upon adsorption on titania and on hydroxyapatite and their relation with biomineralization

The biocompatibility of implant materials used for substitution of bone tissue depends on its ability to induce the deposition of a hydroxyapatite layer when in contact with body fluids. In previous work, some of the authors found that bovine serum albumin (BSA) promotes calcium phosphate deposition...

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Bibliographic Details
Published in:Journal of biomedical materials research 2004-09, Vol.70A (3), p.420-427
Main Authors: Serro, A. P., Bastos, M., Pessoa, J. Costa, Saramago, B.
Format: Article
Language:English
Subjects:
Adsorption
Animals
Biocompatible Materials - chemistry
biomineralization
bovine serum albumin (BSA) adsorption
Calorimetry, Differential Scanning
Cattle
Circular Dichroism
conformational changes
hydroxyapatite
Hydroxyapatites - chemistry
Protein Conformation
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - metabolism
titania
Titanium - chemistry
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Summary:The biocompatibility of implant materials used for substitution of bone tissue depends on its ability to induce the deposition of a hydroxyapatite layer when in contact with body fluids. In previous work, some of the authors found that bovine serum albumin (BSA) promotes calcium phosphate deposition if preadsorbed on hydroxyapatite and retards precipitation if preadsorbed on titania. In the present study, we investigated the adsorption of BSA upon particles of titania and hydroxyapatite in order to understand the different role played by the protein on the mineralization of both biomaterials. The adsorption isotherms were determined and the structural changes induced by adsorption at different surface coverages were investigated by circular dichroism spectroscopy and differential scanning microcalorimetry. At low surface coverages, the adsorbed BSA molecules lost part of their α‐helix content. However, at high surface coverages, corresponding to the plateau values of the adsorption isotherms, the BSA molecules did not undergo structural rearrangements upon adsorption. In the latter circumstances, the availability of BSA calcium binding sites, which should be responsible for inducing mineralization, depends on the electrostatic interactions between BSA and the sorbent surface. A possible explanation for the different mineralization behavior of hydroxyapatite and titania is advanced. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 420–427, 2004
ISSN:1549-3296
0021-9304
1552-4965
1097-4636
DOI:10.1002/jbm.a.30096