Lateral ABA transport in maize roots (Zea mays): visualization by immunolocalization

The intensity of an ABA (abscisic acid) signal as a root-to-shoot signal, as well as its action on root hydraulic conductivity, strongly depends on the distribution of ABA during its radial transport across roots. Therefore ABA was visualized by immunolocalization with monoclonal ABA antibodies unde...

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Bibliographic Details
Published in:Journal of experimental botany 2004-08, Vol.55 (403), p.1635-1641
Main Authors: Schraut, D, Ullrich, C.I, Hartung, W
Format: Article
Language:English
Subjects:
ABA immunolocalization
abscisic acid
Abscisic Acid - metabolism
active transport
Antibodies
Antibodies, Monoclonal - chemistry
Biological and medical sciences
Biological Transport
Cell physiology
cell walls
Corn
Endodermis
exodermis
Fluorescence
Fundamental and applied biological sciences. Psychology
Hypodermis
immunohistochemistry
Immunohistochemistry - methods
lateral ABA transport
maize root sections
mesocotyls
Microscopy
Monoclonal antibodies
Plant physiology and development
Plant roots
Plant Roots - metabolism
Plasma membrane and permeation
Research Papers: Cell and Molecular Biology, Biochemistry and Molecular Physiology
root cap
root meristems
roots
Seedlings
Signal Transduction
Water flow
Xylem
Zea mays
Zea mays - metabolism
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Summary:The intensity of an ABA (abscisic acid) signal as a root-to-shoot signal, as well as its action on root hydraulic conductivity, strongly depends on the distribution of ABA during its radial transport across roots. Therefore ABA was visualized by immunolocalization with monoclonal ABA antibodies under conditions of lateral water flow induced by the application of a pressure gradient to the cut surface of the mesocotyl of maize seedlings. From the labelling of rhizodermis, hypodermis, cortical cells, and endodermis of roots of hydroponically (no exodermis) and aeroponically (with exodermis) grown seedlings it is concluded that the exodermis acts as a barrier to apoplastic transport that controls ABA uptake and efflux, but that the endodermis can easily be overcome via an apoplastic bypass. In longitudinal sections the strongest ABA signals originated from the root cap and the meristematic root tip, which is in agreement with the non-vacuolated cells of these tissues being an effective anion trap for ABA.
ISSN:0022-0957
1460-2431
1460-2431
DOI:10.1093/jxb/erh193