Probing young drinking water biofilms with hard and soft particles

• Published in: Water research (Oxford) 2009, Vol.43 (1), p.117-126
• Main Authors: Paris, Tony, Skali-Lami, Salaheddine, Block, Jean-Claude
• Format: Article
• Language: English
• Subjects: Accumulation; Applied sciences; Biofilm; Biofilms - growth & development; Drinking water; Escherichia coli; Escherichia coli - cytology; Escherichia coli - physiology; Exact sciences and technology; fungi; Green Fluorescent Proteins - metabolism; Hardness; microbial contamination; Microspheres; Other industrial wastes. Sewage sludge; Particles; pipes; Pollution; polystyrenes; Polystyrenes - metabolism; Shear Strength; shear stress; Time Factors; Wall shear rate; Wastes; Water Supply; Water treatment and pollution
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2008.10.009

The aim of our study was to investigate, through the use of soft ( Escherichia coli) and hard (polystyrene microspheres) particles, the distribution and persistence of allochthonous particles inoculated in drinking water flow chambers. Biofilms were allowed to grow for 7–10 months in tap water from Nancy's drinking water network and were composed of bacterial aggregates and filamentous fungi. Both model particles adhered almost exclusively on the biofilms ( i.e. on the bacterial aggregates and on the filamentous structures) and not directly on the uncolonized walls (glass or Plexiglas). Biofilm age ( i.e. bacterial density and biofilm properties) and convective-diffusion were found to govern particle accumulation: older biofilms and higher wall shear rates both increased the velocity and the amount of particle deposition on the biofilm. Persistence of the polystyrene particles was measured over a two-month period after inoculation. Accumulation amounts were found to be very different between hard and soft particles as only 0.03‰ of the soft particles inoculated accumulated in the biofilm against 0.3–0.8% for hard particles.