In vitro antimicrobial effect of bacteriophages on human dentin infected with Enterococcus faecalis ATCC 29212

This study assessed the effect of bacteriophages on the viability of Enterococcus faecalis. Human dental roots were inoculated with a suspension of E. faecalis at three different multiplicities of infection − 0.1, 1.0 and 10.0. The phage lysate was able to significantly inhibit bacteria growth when...

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Bibliographic Details
Published in:Oral microbiology and immunology 2004-10, Vol.19 (5), p.327-330
Main Authors: Paisano, A. F., Spira, B., Cai, S., Bombana, A. C.
Format: Article
Language:English
Subjects:
Bacteriology
bacteriophage
Bacteriophages
Biological and medical sciences
Colony Count, Microbial
Dental Pulp Cavity - microbiology
Dentin - microbiology
Dentistry
endodontic infection
Enterococcus faecalis
Enterococcus faecalis - virology
Fundamental and applied biological sciences. Psychology
Gram-Positive Bacterial Infections - therapy
Humans
Microbiology
Miscellaneous
phage
phage therapy
Virology
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Summary:This study assessed the effect of bacteriophages on the viability of Enterococcus faecalis. Human dental roots were inoculated with a suspension of E. faecalis at three different multiplicities of infection − 0.1, 1.0 and 10.0. The phage lysate was able to significantly inhibit bacteria growth when incubated at the multiplicities of infection of 1.0, 10.0 and 0.1. The dental roots were also inoculated with bacteria for 6 days to allow bacterial penetration into the teeth tubules. Addition of the phage lysate to the roots following the 6‐day incubation period led to a substantial reduction in bacteria viability. Phage therapy may be an important alternative for the treatment of root canal infections refractory to conventional endodontic therapy.
ISSN:0902-0055
1399-302X
DOI:10.1111/j.1399-302x.2004.00166.x