Differential transplantability of tumor-associated stromal cells

At the time of transplantation, tumor fragments contain "passenger" cells: endothelial cells and other stromal cells from the original host. Here, we investigated the fate of genetically labeled endothelial and nonendothelial stromal cells after transplantation in syngeneic mice. We report...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2004-09, Vol.64 (17), p.5920-5924
Main Authors: DUDA, Dan G, FUKUMURA, Dai, MUNN, Lance L, BOOTH, Michael F, BROWN, Edward B, PEIGEN HUANG, SEED, Brian, JAIN, Rakesh K
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Cell Division - physiology
Cell Survival - physiology
Endothelium, Vascular - metabolism
Endothelium, Vascular - pathology
Mammary Neoplasms, Experimental - blood supply
Mammary Neoplasms, Experimental - metabolism
Mammary Neoplasms, Experimental - pathology
Medical sciences
Mice
Mice, Transgenic
Neoplasm Transplantation
Neovascularization, Pathologic - metabolism
Neovascularization, Pathologic - pathology
Pharmacology. Drug treatments
Stromal Cells - metabolism
Stromal Cells - pathology
Tumors
Vascular Endothelial Growth Factor A - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:At the time of transplantation, tumor fragments contain "passenger" cells: endothelial cells and other stromal cells from the original host. Here, we investigated the fate of genetically labeled endothelial and nonendothelial stromal cells after transplantation in syngeneic mice. We report that angiogenic stroma associated with tumor or adipose tissue persists when transplanted, remains functional, and governs the initial neovascularization of grafted tissue fragments for more than 4 weeks after implantation. Surprisingly, the passenger endothelial cells survive longer than other stromal cells, which are replaced by host-activated fibroblasts after 3 weeks. The transplantability of tumor stroma suggests that the angiogenic potential of a tumor xenograft, which determines its viability, depends on the presence of passenger endothelial cells and other stromal cells within the xenograft. These studies of tumor tissue transplantation provide a platform for exploring the role of epithelial-stromal interactions in studies of tumor heterogeneity and drug resistance.
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.can-04-1268