Simultaneous Maintenance of Human Cord Blood SCID‐Repopulating Cells and Expansion of Committed Progenitors at Low O2 Concentration (3%)

In the present work, we tested the hypothesis that liquid cultures (LCs) of cord blood CD34+ cells at an appropriate low O2 concentration could simultaneously allow colony‐forming cell (CFC) expansion and nonobese diabetic/severe combined immunodeficiency mice–repopulating cell (SRC) maintenance. We...

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Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2004, Vol.22 (5), p.716-724
Main Authors: Ivanovic, Zoran, Hermitte, Francis, de la Grange, Philippe Brunet, Dazey, Bernard, Belloc, Francis, Lacombe, Francis, Vezon, Gérard, Praloran, Vincent
Format: Article
Language:English
Subjects:
Animals
Antigens, CD34 - immunology
Antigens, Surface - immunology
Cell Culture Techniques - methods
Cell Differentiation
Cell Hypoxia - immunology
Cell Proliferation
Cells, Cultured
Cord blood
Cord Blood Stem Cell Transplantation - methods
Ex vivo expansion
Female
Fetal Blood - immunology
Fetal Blood - metabolism
Hematopoiesis - immunology
Hematopoietic Stem Cells - immunology
Hematopoietic Stem Cells - metabolism
Humans
Hypoxia
IL‐3
Infant, Newborn
Mice
Mice, SCID
NOD/SCID Stem cells
Oxygen Consumption - immunology
Phenotype
Pregnancy
Severe combined immunodeficiency–repopulating cells
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Summary:In the present work, we tested the hypothesis that liquid cultures (LCs) of cord blood CD34+ cells at an appropriate low O2 concentration could simultaneously allow colony‐forming cell (CFC) expansion and nonobese diabetic/severe combined immunodeficiency mice–repopulating cell (SRC) maintenance. We first found that 3% was the minimal O2 concentration, still allowing the same rate of CFC expansion as at 20% O2. We report here that 7‐day LCs of cord blood CD34+ cells at 3% O2 maintain SRC better than at 20% O2 and allow a similar amplification of CFCs (35‐ to 50‐fold) without modifying the CD34+ cell proliferation. Their phenotypic profile (antigens: HLA‐DR, CD117, CD33, CD13, CD11b, CD14, CD15, and CD38) was not modified, with exception of CD133, whose expression was lower at 3% O2. These results suggest that low O2 concentrations similar to those found in bone marrow participates in the regulation of hematopoiesis by favoring stem cell–renewing divisions. This expansion method that avoids stem cell exhaustion could be of paramount interest in hematopoietic transplantation by allowing the use of small‐size grafts in adults.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.22-5-716