Instrumentation for advanced microseparations in pharmaceutical analysis and proteomics

Small-volume chromatographic columns are only able to generate narrow peaks when flow rates, injection volume and instrument components, such as detector, connecting tubing and fittings, are matched to the peak dispersion from the column. Criteria for the proper design of chromatographic instrumenta...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemical and biophysical methods 2004-09, Vol.60 (3), p.233-263
Main Authors: Rozing, Gerard, Nägele, Edgar, Hörth, Patric, Vollmer, Martin, Moritz, Ralf, Glatz, Bernd, Gratzfeld-Hüsgen, Angelika
Format: Article
Language:English
Subjects:
Capillary HPLC
Chromatography, High Pressure Liquid - instrumentation
Chromatography, Ion Exchange - instrumentation
Chromatography, Liquid - instrumentation
Column switching
Electronic flow control
Mass spectrometry
Nanoflow HPLC
Peak dispersion
Proteomics
Spectrometry, Mass, Electrospray Ionization - instrumentation
Superficially porous particles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Small-volume chromatographic columns are only able to generate narrow peaks when flow rates, injection volume and instrument components, such as detector, connecting tubing and fittings, are matched to the peak dispersion from the column. Criteria for the proper design of chromatographic instrumentation are therefore derived from a general model on total dispersion. The performance of such a system is then experimentally evaluated from applications run on narrow-bore, small-volume columns. In order to achieve flow rates that match the dimensions of such columns, a new concept for electronic flow control (EFC) is introduced. A theoretical optimization of column efficiency and throughput is discussed and the results verified with practical examples on short, narrow-bore columns packed with small, porous and superficially porous particles. For complex sample mixtures, the concept of peak capacity is introduced and applied to orthogonal separation principles in multiple chromatographic dimensions through column switching techniques.
ISSN:0165-022X
1872-857X
DOI:10.1016/j.jbbm.2004.01.005