Separation of racemic 2,4-dinitrophenyl amino acids on zirconia-immobilized quinine carbamate in reversed-phase liquid chromatography

Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate‐coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP‐amino acids in reversed‐phase liquid chromato...

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Bibliographic Details
Published in:Journal of separation science 2004-08, Vol.27 (12), p.977-982
Main Authors: Park, Jung Hag, Lee, Joon Woo, Song, Young Tae, Ra, Chun Sup, Cha, Jin Soon, Ryoo, Jae Jeong, Lee, Wonjae, Kim, In Whan, Jang, Myung Duk
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Analytical chemistry
Carbamates - chemistry
Chemistry
Chiral stationary phase
Chromatographic methods and physical methods associated with chromatography
Chromatography - methods
Chromatography, Liquid - methods
Dinitrobenzenes - chemistry
DNP-amino acids
Exact sciences and technology
Hydrogen-Ion Concentration
Other chromatographic methods
Quinine - chemistry
Quinine carbamate
Reversed-phase liquid chromatography
Silicon - chemistry
Stereoisomerism
Temperature
Time Factors
Zirconia
Zirconium - chemistry
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Summary:Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate‐coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP‐amino acids in reversed‐phase liquid chromatography. Retention and enantioselectivity of this phase were compared to those for quinine carbamate bonded onto silica. Most amino acids studied were separated on the quinine carbamate‐zirconia CSP although retention was longer and chiral selectivity was somewhat lower than on the corresponding silica CSP. Increased retention and decreased selectivity are probably due to strong non‐enantioselective Lewis acid‐base interactions between the amino acid molecule and the residual Lewis acid sites on the zirconia surface.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200301713