Towards More Realistic In Vitro Release Measurement Techniques for Biodegradable Microparticles

• Published in: Pharmaceutical research 2009-03, Vol.26 (3), p.691-699
• Main Authors: Klose, D, Azaroual, N, Siepmann, F, Vermeersch, G, Siepmann, J
• Format: Article
• Language: English
• Subjects: agarose gel; Biochemistry; Biocompatible Materials - chemistry; Biodegradable materials; Biological and medical sciences; Biological Transport; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Cell culture; diffusion; Drug Carriers - chemistry; Drug delivery systems; General pharmacology; Lactic Acid - chemistry; Magnetic Resonance Spectroscopy; Measurement techniques; Medical Law; Medical sciences; microparticles; Microscopy, Electron, Scanning; Models, Theoretical; Nanoparticles; Particle Size; Pharmaceutical Preparations - administration & dosage; Pharmaceutical sciences; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Pharmacology/Toxicology; Pharmacy; PLGA; Polyglycolic Acid - chemistry; Polymers; Propranolol - administration & dosage; Propranolol - pharmacokinetics; release test; Research Paper; Sepharose - chemistry; Surface Properties
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-008-9747-4

Purpose To better understand the importance of the environmental conditions for drug release from biodegradable microparticles allowing for the development of more appropriate in vitro release measurement techniques. Methods Propranolol HCl diffusion in various agarose gels was characterized by NMR and UV analysis. Fick's law was used to theoretically predict the mass transport kinetics. Drug release from PLGA-based microparticles in such agarose gels was compared to that measured in agitated bulk fluids (“standard” method). Results NMR analysis revealed that the drug diffusivity was almost independent of the hydrogel concentration, despite of the significant differences in the systems' mechanical properties. This is due to the small size of the drug molecules/ions with respect to the hydrogel mesh size. Interestingly, the theoretically predicted drug concentration-distance-profiles could be confirmed by independent experiments. Most important from a practical point of view, significant differences in the release rates from the same batch of PLGA-based microparticles into a well agitated bulk fluid versus a semi-solid agarose gel were observed. Conclusion Great care must be taken when defining the in vitro conditions for drug release measurements from biodegradable microparticles. The obtained new insight can help facilitating the development of more appropriate in vitro release testing procedures.