The role of electron transport in the defence response of the South African abalone, Haliotis midae

In order to establish health management systems for farmed abalone, it is necessary to understand how the abalone immune system functions and responds to stimulation. Two electron transport system genes, cytochrome b and cytochrome c oxidase III, were found to be upregulated in a cDNA microarray exp...

Full description

Saved in:
Bibliographic Details
Published in:Fish & shellfish immunology 2009, Vol.26 (1), p.171-176
Main Authors: van Rensburg, Marike Janse, Coyne, Vernon E.
Format: Article
Language:English
Subjects:
Abalone
Adenosine Triphosphate - metabolism
Animals
Antimycin A - pharmacology
Bacteria - immunology
Cell Survival - drug effects
Electron transport
Electron Transport - immunology
Gastropoda - drug effects
Gastropoda - immunology
Haliotis
Haliotis midae
Hemocytes - drug effects
Hemocytes - microbiology
Immune system
Phagocytosis - drug effects
Superoxides - metabolism
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In order to establish health management systems for farmed abalone, it is necessary to understand how the abalone immune system functions and responds to stimulation. Two electron transport system genes, cytochrome b and cytochrome c oxidase III, were found to be upregulated in a cDNA microarray experiment performed on haemocytes from immune-stimulated abalone (Arendze-Bailey, unpublished). The current study sought to elucidate the role of these genes, and thus the electron transport system, in the abalone immune response by specifically inhibiting cytochrome b with antimycin A and measuring haemocyte immune parameters in vivo. Antimycin A did not decrease haemocyte cell viability, but halved cellular ATP from 4 × 10 12 nM/cell to 2 × 10 12 nM/cell ( p 
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2008.09.016