Atomic force microscopy probing platelet activation behavior on titanium nitride nanocoatings for biomedical applications

Abstract There is increasing interest in developing novel coatings to enhance the biocompatibility of medical implants. A key issue in biocompatibility research is platelet activation and aggregation on the biomaterials' surface. Stoichiometric and nonstoichiometric titanium nitride (TiNx ) fil...

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Bibliographic Details
Published in:Nanomedicine 2009-03, Vol.5 (1), p.64-72
Main Authors: Karagkiozaki, Varvara, MD, MSc, Logothetidis, Stergios, PhD, Kalfagiannis, Nikolaos, MSc, Lousinian, Sylvie, MSc, Giannoglou, Georgios, PhD
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biocompatible Materials - pharmacology
Coated Materials, Biocompatible - pharmacology
Humans
Internal Medicine
Microscopy, Atomic Force - methods
Nanomedicine - methods
Platelet Activation - drug effects
Platelet Adhesiveness - drug effects
Platelets
Spectroscopic ellipsometry
Titanium - pharmacology
Titanium nitride
Wettability
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Summary:Abstract There is increasing interest in developing novel coatings to enhance the biocompatibility of medical implants. A key issue in biocompatibility research is platelet activation and aggregation on the biomaterials' surface. Stoichiometric and nonstoichiometric titanium nitride (TiNx ) films were developed by sputtering as case study materials, for probing platelet activation behavior onto them. Atomic force microscopy (AFM) facilitates the real-time studies of cells and guarantees cellular viability. In this work a methodology for platelets study by AFM was developed. The morphological, structural, optical, and wettability properties of the TiNx films were obtained by AFM, x-ray diffraction, spectroscopic ellipsometry, and contact angle measurements.The properties of TiNx films were correlated with their thrombogenicity involving platelets' adhesion, activation and protein clustering mechanisms. It was found that the TiNx films stoichiometry and surface roughness affect the platelet response. The stoichiometric and smoother TiN films promote platelets adhesion and activation.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2008.07.005