The POU Factor Oct-25 Regulates the Xvent-2B Gene and Counteracts Terminal Differentiation in Xenopus Embryos

The Xvent-2B promoter is regulated by a BMP-2/4-induced transcription complex comprising Smad signal transducers and specific transcription factors. Using a yeast one-hybrid screen we have found that Oct-25, a Xenopus POU domain protein related to mammalian Oct-3/4, binds as an additional factor to...

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Bibliographic Details
Published in:The Journal of biological chemistry 2004-10, Vol.279 (42), p.43735-43743
Main Authors: Cao, Ying, Knöchel, Sigrun, Donow, Cornelia, Miethe, Josef, Kaufmann, Eckhard, Knöchel, Walter
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Blastomeres - cytology
Blastomeres - physiology
Cell Differentiation
Cloning, Molecular
Deoxyribonuclease I
DNA Footprinting
DNA Primers
Embryo, Nonmammalian - physiology
Freshwater
Gene Expression Regulation, Developmental - physiology
Genes, Reporter
Homeodomain Proteins - genetics
Molecular Sequence Data
Promoter Regions, Genetic - genetics
Transcription Factors - genetics
Transcription Factors - metabolism
Xenopus laevis - embryology
Xenopus laevis - genetics
Xenopus Proteins - genetics
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Summary:The Xvent-2B promoter is regulated by a BMP-2/4-induced transcription complex comprising Smad signal transducers and specific transcription factors. Using a yeast one-hybrid screen we have found that Oct-25, a Xenopus POU domain protein related to mammalian Oct-3/4, binds as an additional factor to the Xvent-2B promoter. This interaction was further confirmed by both in vitro and in vivo analyses. The Oct-25 gene is mainly transcribed during blastula and gastrula stages in the newly forming ectodermal and mesodermal germ layers. Luciferase reporter gene assay demonstrated that Oct-25 stimulates transcription of the Xvent-2B gene. This stimulation depends on the Oct-25 binding site and the bone morphogenetic protein-responsive element. Furthermore, Oct-25 interacts in vitro with components of the Xvent-2B transcription complex, like Smad1/4 and Xvent-2. Overexpression of Oct-25 results in anterior/posterior truncations and lack of differentiation for neuroectoderm- and mesoderm-derived tissues including blood cells. This effect is consistent with an evolutionarily conserved role of class V POU factors in the maintenance of an undifferentiated cell state. In Xenopus , the molecular mechanism underlying this process might be coupled to the expression of Xvent proteins.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M407544200