Cellular and subcellular localization of the inhibitory glycine receptor in hippocampal neurons

Inhibitory glycine receptors are most abundant in spinal cord and brainstem, and glycinergic synapses have a well-established role in the regulation of locomotor behavior. Little is known about the function of glycine receptors in cortex and hippocampus, where GABA plays a dominant role in synaptic...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-11, Vol.324 (3), p.1137-1142
Main Authors: Brackmann, Marian, Zhao, Congjian, Schmieden, Volker, Braunewell, Karl-Heinz
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Brain Stem - metabolism
Calcium - chemistry
Calcium - metabolism
Calcium-binding protein
Cerebral Cortex - metabolism
Dimerization
Electrophoresis, Polyacrylamide Gel
GAD
Glycine - chemistry
Glycine receptor
Hippocampal neurons
Hippocampus - metabolism
Immunocytochemistry
Immunohistochemistry
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - metabolism
Neurocalcin
Neurons - metabolism
Protein Binding
Protein Conformation
Protein Structure, Tertiary
Rats
Rats, Wistar
Receptors, Calcium-Sensing - biosynthesis
Receptors, Calcium-Sensing - metabolism
Receptors, Glycine - chemistry
Receptors, Glycine - metabolism
Spinal Cord - metabolism
Synapsin I
Synapsins - metabolism
Tissue Distribution
VILIP-1
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Inhibitory glycine receptors are most abundant in spinal cord and brainstem, and glycinergic synapses have a well-established role in the regulation of locomotor behavior. Little is known about the function of glycine receptors in cortex and hippocampus, where GABA plays a dominant role in synaptic inhibition. Therefore, we have investigated tissue and cellular expression of glycine receptor α-subunits. Western blot and immunohistochemical analyses reveal the presence of glycine receptors in hippocampal tissue. Immunocytochemical experiments in hippocampal cultures show prominent cellular expression of glycine receptors in pyramidal neurons and GAD-positive interneurons similar to the calcium-binding protein VILIP-1 with widespread hippocampal distribution. On the subcellular level we found co-staining of GlyR and the presynaptic marker synapsin I. Furthermore, co-staining with GAD at synaptic terminals indicated partial co-localization of GABA- and glycine receptors.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.09.172