Immunohistochemical study of apparently intact coronary artery in a child after Kawasaki disease

• Published in: Pediatrics international 2004-10, Vol.46 (5), p.590-596
• Main Authors: Suzuki, Atsuko, Miyagawa-Tomita, Sachiko, Komatsu, Keiko, Nakazawa, Makoto, Fukaya, Takashi, Baba, Kunizou, Yutani, Chikao
• Format: Article
• Language: English
• Subjects: Child, Preschool; Coronary Artery Disease - diagnosis; Coronary Artery Disease - etiology; Coronary Artery Disease - metabolism; Coronary Vessels - chemistry; Coronary Vessels - pathology; Humans; Immunohistochemistry - methods; inducible nitric oxid synthesis; Infant; Kawasaki disease; Mucocutaneous Lymph Node Syndrome - complications; Nitric Oxide Synthase - analysis; Nitric Oxide Synthase Type II; normal coronary artery; Platelet-Derived Growth Factor - analysis; Receptors, Transforming Growth Factor beta - analysis; Time Factors; Transforming Growth Factor beta - analysis; transforming growth factor-β-1; Vascular Endothelial Growth Factor A - analysis
• ISSN: 1328-8067; 1442-200X
• DOI: 10.1111/j.1442-200x.2004.01943.x

Background : Coronary arterial lesions (CAL) due to Kawasaki disease (KD) often show progressive intimal hyperplasia even many years after the disease. However, most patients have no CAL after the acute phase, and it is an important issue whether or not coronary arteries without CAL have significant intimal hyperplasia, and whether or not there is the potential for this to progress to stenosis and/or atherosclerosis. Methods : The authors examined formalin‐fixed specimens of the coronary arteries immunohistochemically, using antibodies against vascular growth factors (GFs), the receptors of transforming growth factor‐β (TβRs) and inducible nitric oxid synthesis (iNOS) in a KD patient without CAL, and also in four control patients: two with CAL due to KD and two without a history of KD. Results : Vascular endothelial GFs, Platelet‐derived growth factor‐A (PDGF‐A) and TβRs were expressed in the vascular smooth muscle cells of all patients. PDGF‐A, transforming Gfβ1 and iNOS were expressed in the intimal smooth muscle cells of the KD but not the normal coronary artery without a history of KD. The number of TβR‐II‐positive cells were fewer than TβR‐I‐positive cells in the intima of CAL due to KD, but the number was of both almost same in the intima of coronary artery without CAL after KD and in the normal coronary. Conclusion : The intact coronary artery 13 months after KD still showed the influence of the inflammation of KD. Although the authors speculate that the intimal proliferation will not continue beyond the acute phase, those patients may have a risk factor for atherosclerosis.