Synthetic sulfonolipids deduced from sulfonoquinovosyl diacylglycerols of sea urchin reduces hepatic ischemia-reperfusion injury in rats

• Published in: Transplantation proceedings 2004-09, Vol.36 (7), p.1965-1969
• Main Authors: Tsuruma, T., Sahara, H., Takenouchi, M., Yagihashi, A., Iwayama, Y., Shima, H., Furuhata, T., Torigoe, T., Hanashima, S., Yamazaki, T., Sugawara, F., Mizushina, Y., Ohta, K., Sakaguchi, K., Sato, N., Hirata, K.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Diglycerides - pharmacology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gastroenterology. Liver. Pancreas. Abdomen; Glycolipids - pharmacology; Liver - blood supply; Liver - drug effects; Liver - pathology; Liver Circulation - drug effects; Liver Function Tests; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Other diseases. Semiology; Rats; Rats, Inbred Lew; Reperfusion Injury - prevention & control; Sea Urchins - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Tissue, organ and graft immunology
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2004.08.089

In hepatic surgery and liver transplantation, ischemia-reperfusion (I/R) is an unavoidable process, and protection against hepatic I/R injury is a major unresolved problem. In this study, we investigated whether 3-O-(6-deoxy-6-sulfono-β-D-glucopyranosyl)-1,2-di-O-acylglycerol bound to saturated C18 fatty acids (β-SQAG9), which was derived from sea urchin intestines, could reduce this injury. This agent was recently reported to have immunosuppressive effects in allogeneic rat skin grafts. Male Lewis rats were divided into two experimental groups. Group 1 rats were injected with SQAG9 (50 mg/kg) into the penile vein 15 minutes before the induction of ischemia and into the portal vein just reperfusion. The same amounts of normal saline were injected into rats in the control group (group 2). Each experimental groups included six rats. Seventy percent hepatic ischemia (20 minutes) was induced by occluding the blood vessels and bile duct with a vascular clamp. For examination of hepatic function, serum levels of aspartate aminotransferase, (AST) alanine transaminase (ALT), and lactic dehydrogenase (LDH) were measured. In addition, histological examination was also assessed. Three hours after reperfusion, the mean plasma concentration of AST, ALT, LDH in group 1 was suppressed compared with group 2. Six hours after reperfusion, the hepatic damage in group 1 was mild in comparison with that in group 2. Our data demonstrated that SQAG-9 reduced the warm hepatic I/R injury.